Crystal structure of azoreductase AzoR from Escherichia coli

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概要

• 論文の詳細を見る

• Azoreductase AzoR is an oxidoreductase isolated from Escherichia coli as an enzyme responsible for the reduction of azo compounds. As the first step toward the elucidation of the molecular mechanism of function, we determined the three-dimensional structure of the enzyme by X-ray crystallography at 1.8 Å resolution. The crystal structure has revealed that AzoR is an FMN-containing and homodimeric enzyme. Each monomer consists of a twisted central parallel β-sheet surrounded on both sides by helices. The overall folding of the protein resembles NQO1, originally called DT-diaphorase [NAD(P)H: quinone reductase, EC 1.6.99.2], a mammalian FAD containing protein without significant sequence identity. (Communicated by Masanori OTSUKA, M.J.A.)

• 日本学士院の論文

著者

• Sasaki Hiroshi

  Department of Biological Technology, Faculty of Science and Engineering,Senshu University of Ishinom

• SAIGO Kaoru

  Department of Biochemistry, Kyushu University School of Medicine

• Kitade Yukio

  Department Of Biomolecular Science Faculty Of Engineering Gifu University

• Tanokura Masaru

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Ito Kosuke

  Department Of Operative Dentistry Okayama University Dental School

• Lee Woo

  Department Of Biological Scienceand Basic Science Research Institute Sungkyunkwan University

• NAKANISHI Masayuki

  Department of Applied Chemistry, Faculty of Engineering, Gifu University

• Zenno Shuhei

  Department Of Biophysics And Biochemistry Graduate School Of Science

• Lee Woo

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers

• Sasaki Hiroshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• ZENNO Shuhei

  Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

• ITO Kosuke

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• NAKANISHI Masayuki

  Department of Biomolecular Science, Faculty of Engineering, Gifu University

• SAIGO Kaoru

  Department Biochemistry, Kyushu University 60 School of Medicine

• LEE Woo

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• SASAKI Hiroshi

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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