Effects of ionization on chemical shifts of the surrounding protons of bromelain inhibitor VI
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概要
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Proton nuclear magnetic resonance (NMR) titration shifts are a very sensitive indication of proton dissociation from carboxyl groups and of hydrogen-bond formation between carboxylate groups and backbone amide protons. As a model protein, we selected bromelain inhibitor VI (BI-VI), which is a unique double-chain molecule (M r = 5888), and characterized almost all the proton resonances in the pH range of 1.5-9.9 by two-dimensional NMR. In this study, we first examined the ionization effects of Asp13H(β-CO2H) and Glu24H(γ-CO 2H) (superscript letters H and L indicate heavy and light chains, respectively). It was found that their ionization affected the chemical shifts of the surrounding protons through a maximum of seven consecutive covalent bonds, but scarcely through non-covalent bonds. Furthermore, it was revealed that the formation of two hydrogen bonds of Ser4L(NH)-Asp9H(β-CO 2H) and Lys19H(NH)-Asp32H(β-CO2H) influenced not only the chemical shifts of the amide protons of Ser4L and Lys19H but also those of the alpha protons of Asp9H and Asp32H. (Communicated by Masanori OTSUKA, M.J.A.)
- 日本学士院の論文
著者
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KOJIMA Masaki
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences
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Tanokura Masaru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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Tanokura Masaru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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HATANO Ken-ichi
Department of Biological Sciences, Faculty of Engineering, Gunma University
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Kojima Masaki
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Hatano Ken-ichi
Department Of Biological Sciences Faculty Of Engineering Gunma University
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