Crystal structure of acylphosphatase from hyperthermophilic archaeon Pyrococcus horikoshii OT3
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概要
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Analysis of the hyperthermophilic archaeon Pyrococcus horikoshii OT3 genome database led to the discovery and cloning of acylphosphatase (ORF PH0305a). To elucidate the first structure of archaeal acylphosphatase, we determined the crystal structure of P. horikoshii acylphosphatase at 1.72 Å resolution. The space group of the crystals was P3221, with unit-cell parameters a = b = 86.6 Å and c = 75.4 Å. The overall fold of P. horikoshii acylphosphatase was very similar to the structures of the eukaryotic enzymes. The conformation of putative active site was highly conserved. (Communicated by Masanori OTSUKA, M.J.A.)
- 日本学士院の論文
著者
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Tanokura Masaru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Miyazono Ken-ichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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Sawano Yoriko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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TANOKURA Masaru
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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MIYAZONO Ken-ichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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