Purification and Some Properties of Two Cellobiohydrolases from Penicillium purpurogenum
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概要
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Two 1,4-β-D-glucan cellobiohydrolases, here called enzymes I and II, were purified by gel filtration, ion-exchange chromatography and chromatofocusing from a culture supernatant of Penicillium purpurogenum. Their homogeneity was verified by polyacrylamide gel disc electrophoresis. The molecular weights of enzymes I and II, estimated by gel filtration, were 84,000 and 64,000,respectively. the enzymes differed in carbohydrate content, amino acid composition and immunological properties.Both enzymes readily hydrolyzed insoluble celluloses such as Avicel, H_3PO_4-swollen cellulose and soluble cello-oligosaccharides with degrees of polymerization higher than 3,but had lower activity towards carboxymethyl cellulose. Both produced cellobiose and a small amount of glucose from insoluble cellulose, but showed minor differences in the hydrolyzates of cello-oligosaccharides and reduced cello-oligosaccharides. A remarkable synergistic action between enzymes I and II was observed in the hydrolysis of Avicel.
- 社団法人日本生物工学会の論文
- 1986-06-25
著者
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Sasaki Hiroshi
Department of Biological Technology, Faculty of Science and Engineering,Senshu University of Ishinom
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TAKAO Shoichi
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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Takao Shoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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KAMAGAWA YOICHI
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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Kamagawa Yoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Sasaki Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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