EFFECTS OF CULTURE CONDITIONS ON HYDROGENASE ACTIVITY OF SCENEDESMUS D_3

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The effects of culture conditions and of culture ages upon hydrogenase activity in Scenedesmus D_3 was investigated with nitrite and benzoquinone as acceptors. It was noticed that level of iron in the culture medium played an important part in the development of hydrogenase activity. The cells grown on a medium of relatively high level of iron (10^<-4>M) showed higher activity both in nitrite and benzoquinone reduction than those grown on a medium of relatively low level of iron (10^<-5>M). In the former case, hydrogenase activity appeared even after a short incubation with hydrogen (30 min). When the cells with low hydrogenase activity were transferred to a medium containing 10^<-4>M iron and the activity was measured every 24 hours, the following facts were observed. (a) Nitrite reduction activity increased markedly during the first 24 hours and then decreased gradually during further cultivation. Furthermore, a parallelism was observed between nitrite reduction activity and cell size. (b) On the other hand, the pattern of change in quinone reduction activity was somewhat different from that of nitrite reduction. This activity appealed only after 3 days' cultivation, reached a maximum at 4th or 5th day and then decreased. However the presence of glucose in the culture medium suppresses the appearance of quinone reduction activity, while the nitrite reduction activity was less markedly affected.
日本植物生理学会の論文