Identification of Medicinal Atractylodes Based on ITS Sequences of nrDNA(Pharmacognosy)

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概要

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• Dried rhizomes of five species of Atractylodes (A. japonica, A, macrocephala, A. lancea, A. chinensis, and A. koreana), Compositae, have been used as crude drugs mainly for the treatment of stomach disorders and for their diuretic properties in Chinese and Japanese traditional medicines. The identification of the botanical origins of these crude drugs is generally difficult from their morphological and chemical features only. In this study, for identification with more reliable, nuclear ribosomal DNA (nrDNA), internal transcribed spacer (ITS) regions of five species of medicinal Atractylodes were sequenced. As a result, specific ITS genotypes were recognized by each species. The four species (A. japonica, A. macrocephala, A. lancea, and A. chinensis) prescribed in Chinese and Japanese Pharmacopoeias as botanical origins of crude Atractylodes drugs could be distinguished by their ITS sequences because they had difference genotypes on the ITS sequences. However, the genotype of A. koreana was the same as that of A. chinensis. Additionally, hybrids between A. lancea and A. chinensis were also recognized as nucleotide additives on their ITS sequences. In this study, several morphological characteristics were researched by their genotype, too. As this result, the hybrids recognized from the genetic analysis had intermediate morphological characteristics between A. lancea and A. chinensis. It was also recognized that A. lancea and A. chinensis except for their hybrids were significant differences. It is therefore suggested that ITS sequences of nrDNA would be useful for the identification of the crude drugs derived from Atractylodes species and their interspecific hybridizations.

• 社団法人日本薬学会の論文
• 2006-02-01

著者

• 竹田 秀一

  ツムラ研究本部

• MIYAMOTO Ken-ichi

  Department of Pharmacology and Pharmaceutics, Graduate School of Natural Science and Technology, Kan

• ABURADA Masaki

  Research Division, Tsumura & Co.

• 油田 正樹

  Res. Inst. Of Pharmaceutical Sciences Musashino Univ.

• 油田 正樹

  Medicinal Evaluation Laboratory Tsumura Research Institute

• Aburada M

  Fac. Of Pharmacy Musashino Univ.

• Aburada M

  Tsumura Research Inst. Pharmacology Ibaraki Jpn

• TAKEDA Shuichi

  Pharmacology Research Department, Central Research Laboratories, R & D Division, Tsumura & Co.

• SHIBA Mao

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• KONDO Kenji

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• MIKI Eiji

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• YAMAJI Hiroki

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• MOROTA Takashi

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• TERABAYASHI Susumu

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• SASAKI Hiroshi

  Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &

• Miyamoto Ken-ichi

  金沢大学 薬学部臨床薬物情報

• Miyamoto Ken-ichi

  Dep. Of Clinical Pharmacy Graduate School Of Natural Sci. And Technol. Kanazawa Univ.

• Kondo Kenji

  Botanical Raw Materials Research Dept. Tsumura & Co.

• Morota Takashi

  Botanical Raw Materials Research Department Tsumura & Co.

• Morota Takashi

  Botanical Raw Materials Research Dept. Tsumura & Co.

• 竹田 秀一

  Tsumura & Co. Tsumura Res. Laboratories

• 油田 正樹

  ツムラ研究本部 医薬評価研究所

• Miyaamoto Ken-ichi

  Department Of Medical Informatics Graduate School Of Medical Science Kanazawa University

• Sasaki Horoshi

  Faculty of Agriculture, Tohoku University

• Morota Takashi

  Pharmacognosy And Medicinal Resources Research Department Central Research Laboratories Tsumura &

• Miyamoto K

  Department Of Hospital Pharmacy School Of Medicine Kanazawa University

• Miyamoto K

  Kanazawa Univ. Hospital Ishikawa Jpn

• Yamaji Hiroki

  Botanical Raw Materials Research Department Tsumura & Co.

• Takeda Shuichi

  Department Of Drug Metabolism & Disposition Tsumura Research Institute Tsumura & Co.

• Miki Eiji

  Pharmacognosy And Medicinal Resources Research Department Central Research Laboratories Tsumura &

• Aburada Masaki

  Musashino Univ. Tokyo Jpn

• Terabayashi Susumu

  Pharmacognosy And Medicinal Resources Research Department Tsumura And Co.

• Terabayashi S

  Tsumura Lab. Ibaraki Jpn

• Terabayashi Susumu

  Pharmacognosy And Medicinal Resources Research Department Central Research Laboratories Tsumura &

• Terabayashi Susumu

  Pharmacognosy Department Tsumura & Co.

• Shiba Mao

  Botanical Raw Materials Research Dept. Tsumura & Co.

• Miyamoto Ken'ichi

  Hospital Pharmacy School Of Medicine Kanazawa University

• Miyamoto Ken-ichi

  Department Of Applied Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University

• Kondo Kenji

  Pharmacognosy And Medicinal Resources Research Department Central Research Laboratories Tsumura &

• Kondo Kenji

  Pharmacognosy Department Tsumura & Co.

• Sasaki Hiroshi

  Japan Kampo Medicine Manufacturers Association

• Sasaki Hiroshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Sasaki Hiroshi

  Pharmaceutical Production Division Chugai Pharmaceutical Co. Ltd.

• Sasaki Hiroshi

  金沢大学 自然科学研究科臨床薬学

• Takeda Shuichi

  Tsumura & Co. Tsumura Research Laboratories

• Miyamoto Ken-ichi

  Department of Clinical Pharmacy, Graduate School of Natural Science and Technology, Kanazawa University

• Sasaki Hiroshi

  Pharmaceutical Institute, Tohoku University

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