Adhesive Interaction of Highly Malignant Hepatoma AH66F Cells with Mesothelial Cells
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the mechanism of adhesion of highly malignant ascites hepatoma AH66F cells to mesothelial cells. The adhesion rate of AH66F cells to mesentery-derived mesothelial cells (M-cells) was about 46% at 37℃, but it decreased to about 27% at 4℃. The adhesion rate of AH66F cells was about 25% in the presence of leukocyte function-associated antigen 1 (LFA-1) mAb at both 4℃ and 37℃. When M-cells were treated with hyaluronidase, the AH66F/M-cell adhesion was decreased to half at 37℃ and had nearly disappeared at 4℃. The residual adhesion of AH66F cells to M-cells treated with hyaluronidase almost disappeared in the presence of LFA-1 mAb. AH66F cells strongly adhered to a hyaluronate (HA)-coated plate, but not to a bovine serum albumin-coated plate. AF66F cells expressed a CD44 molecule (a HA receptor) in the plasma membrane, with a molecular size of about 85 to 90 kDa, corresponding to the CD44H isoform.These results indicated that the adhesion of AH66F cells to mesothelial cells is composed of pathways of CD44/HA and LFA-1/ICAM-1.
- 1999-07-15
著者
-
NOMURA Masaaki
Department of Pharmacology and Pharmaceutics, Graduate School of Natural Science and Technology, Kan
-
SUGIURA Yuhki
Department of Pharmacology and Pharmaceutics, Graduate School of Natural Science and Technology, Kan
-
TATSUMI Yasuaki
Department of Pharmacology and Pharmaceutics, Graduate School of Natural Science and Technology, Kan
-
MIYAMOTO Ken-ichi
Department of Pharmacology and Pharmaceutics, Graduate School of Natural Science and Technology, Kan
-
Nomura Masaaki
北陸大学 薬学部臨床薬理学
-
Nomura Masaaki
北陸大学 薬学部合成化学
-
Miyamoto K
Department Of Hospital Pharmacy School Of Medicine Kanazawa University:department Of Medicinal Infor
-
Nomura Masaaki
Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
-
Sugiura Yuhki
Hospital Pharmacy Kanazawa University
-
Tatsumi Yasuaki
Department Of Legal Medicine Kanazawa University School Of Medicine
-
Tatsumi Yasuaki
Department Of Medical Technology Nagoya University School Of Health Sciences
-
Tatsumi Y
Department Of Medical Technology Nagoya University School Of Health Sciences
-
Miyamoto Ken-ichi
Department Of Applied Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
-
Noguchi Masamichi
Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
-
Nomura Masaaki
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University
関連論文
- Adhesive Interaction of Highly Malignant Hepatoma AH66F Cells with Mesothelial Cells
- Tectorigenin, an Isoflavone of Pueraria thunbergiana BENTH., Induces Differentiation and Apoptosis in Human Promyelocytic Leukemia HL-60 Cells
- Differentiation-Inducing Effect of Magnolialide, a 1β-Hydroxyeudesmanolide Isolated from Cichorium intybus, on Human Leukemia Cells
- Studies on Constituents with Cytotoxic Activity from the Stem Bark of Syringa velutina
- Effect of thalidomide on endotoxin-induced decreases in activity and expression of hepatic cytochrome P450 3A2
- The influence of the sennosides on absorption of glycyrrhetic acid in rats
- Effects of Rikkunshi-to, a Traditional Japanese Medicine, on the Delay of Gastric Emptying Induced by N^G-Nitro-L-arginine
- Effects of Rikkunshi-to, a Traditional Japanese Medicine, on the Delay of Gastric Emptying Induced by NG-Nitro-L-arginine
- Structure Activity Relationships of Quinoxalin-2-one Derivatives as Platelet-Derived Growth Factor-β Receptor (PDGFβ R) Inhibitors, Derived from Molecular Modeling
- Convenient synthetic method for 3-(3-substituted indol-2-yl)quinoxalin-2- ones as VEGF inhibitor