Ca2+ Ionophore and Phorbol Ester Stimulate Diacylglycerol Formation and Phosphatidylcholine Hydrolysis in Rat Parotid Acinar Cells.
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概要
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We investigated the effects of A23187 and phorbol 12, 13-dibutyrate (PDBu) on sn-1, 2-diacylglycerol (DAG) accumulation and phosphatidylcholine (PC) hydrolysis in rat parotid acinar cells. Both A23187 and PDBu, in concentration ranges of 0.001-0.1 μM, stimulated DAG accumulation and PC hydrolysis in a time- and concentration-dependent manner. Treatment with A23187 and PDBu stimulated the release of [<SUP>3</SUP>H]choline and [<SUP>3</SUP>H]phosphocholine into the medium, indicating [<SUP>3</SUP>H]PC hydrolysis is due to the activation of phospholipases C and D; however, [<SUP>3</SUP>H]phosphatidylethanolamine hydrolysis was not indicated. These releases were unaffected by the addition of glucose 6-phosphate, a phosphatase inhibitor. Staurosporine, a protein kinase C inhibitor, significantly inhibited the DAG accumulation and the PC hydrolysis stimulated by these agents. Combinations of A23187 and PDBu potentiated the stimulatory effect which each of these agents alone had on DAG accumulation and PC hydrolysis. This mode of action was additive but not synergistic. These results suggest that DAG accumulation induced by A23187 and PDBu is related to the PC hydrolysis mediated via the activation of phospholipases C and D, and that it is not related to phosphatidylethanolamine hydrolysis.
- 公益社団法人 日本薬理学会の論文
著者
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Izawa Tetsuya
Laboratory For Exercise And Applied Physiology Tokyo College Of Pharmacy
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Suda Kazuhiro
Department Of Human System Science Graduate School Of Decision Science And Technology Tokyo Institut
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Tsuboi Minoru
Department Of Pharmacology Tokyo College Of Pharmacy
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Noguchi Masamichi
Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
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KOMABAYASHI Takao
Department of Human System Science, Graduate School of Decision Science and Technology, Tokyo Institute of Technology
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Izawa Tetsuya
Laboratory for Exercise and Applied Physiology, Tokyo College of Pharmacy
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Yakata Atsushi
Department of Physiology and Pharmacology, Tokyo College of Pharmacy
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Komabayashi Takao
Department of Physiology and Pharmacology, Tokyo College of Pharmacy
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