Insulin Signaling in Adipocytes Differentiated from Mouse Stromal MC3T3-G2/PA6 Cells(Biochemistry)
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概要
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The stromal MC3T3-G2/PA6 (PA6) cells from mouse clavaria did not require insulin for differentiation into mature adipose cells, although insulin is well known to play a key role in adipocyte differentiation. Large lipid droplets were observed in the cytoplasm of PA6 cells, and mRNA expression of the adipose specific proteins (aP2, PPARγ, C/EBPα, FAS, GLUT4, leptin, and adiponectin) as differentiation markers appeared or increased clearly in the cells at 8d after stimulation without insulin. In addition, the glycerol released from the cells (lipolysis) was increased in a concentration-dependent manner by isoproterenol. However, the isoproterenol-induced lipolysis in the cells was not influenced by treatment with insulin, although that was observed in extramedullary adipocytes, 3T3-L1 cells. On the other hand, the 2-deoxy-D-[1-^3H]glucose uptake in differentiated PA6 cells also increased by insulin, as shown in other adipose cells. In the cells, insulin induced the phosphorylation of extracellular signal-regulated kinases (Erks), Akt at Ser 473 and ribosomal p70 S6 protein kinase (p70 S6K) at Thr 389, and the insulin-induced 2-deoxy-D-[1-^3H]glucose uptake was inhibited by pre-treatment with wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), or ML-9, an Akt inhibitor. These results suggest that the insulin signal for adipogenesis (lipogenesis) and lipolysis in bone marrow stroma PA6 cells differs from extramedullary adipocytes, such as 3T3-L1 cells.
- 公益社団法人日本薬学会の論文
- 2005-11-01
著者
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MIYAMOTO Kenichi
Department of Medical Genetics, Ehime University School of Medicine
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Igarashi Manabu
Department Of Health For Senior Citizens Division Of Preventive Medicine Social Medicine Cluster Hok
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Nomura Masaaki
北陸大学 薬学部臨床薬理学
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Nomura Masaaki
北陸大学 薬学部合成化学
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YOKOGAWA Koichi
Department of Hospital Pharmacy, School of Medicine, Kanazawa University
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Miyamoto K
Department Of Hospital Pharmacy School Of Medicine Kanazawa University:department Of Medicinal Infor
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Nomura Masaaki
Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
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Igarashi Manabu
Department Of Hospital Pharmacy School Of Medicine Kanazawa University
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MORITO Satoshi
Department of Clinical Pharmacy, Graduate School of Natural Science and Technology, Kanazawa Univers
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YAGUCHI Kuniko
Department of Clinical Pharmacy, Graduate School of Natural Science and Technology, Kanazawa Univers
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IMADA Mikiko
Department of Hospital Pharmacy, School of Medicine, Kanazawa University
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TACHIKAWA Chihiro
Department of Hospital Pharmacy, School of Medicine, Kanazawa University
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NOMURA Masaaki
The Faculty of Pharmaceutical Sciences, Hokuriku University
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MORITANI Shuzo
The Faculty of Nursing and Welfare Sciences, Fukui Prefectural University
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Miyamoto Kenichi
Department Of Hospital Pharmacy And Department Of Medicinal Informatics Graduate School Of Medical S
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Imada Mikiko
Department Of Hospital Pharmacy School Of Medicine Kanazawa University
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Yaguchi Kuniko
Department Of Clinical Pharmacy Graduate School Of Natural Science And Technology Kanazawa Universit
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Morito Satoshi
Department Of Clinical Pharmacy Graduate School Of Natural Science And Technology Kanazawa Universit
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Moritani Shuzo
Department Of Nursing Faculty Of Nursing And Welfare Fukui Prefectural University
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Yokogawa Koichi
Department Of Hospital Pharmacy School Of Medicine Kanazawa University:department Of Medicinal Infor
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Yokogawa Koichi
Department Of Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Tachikawa Chihiro
Department Of Hospital Pharmacy School Of Medicine Kanazawa University
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Noguchi Masamichi
Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
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Miyamoto Kenichi
Department Of Chemistry For Materials Faculty Of Engineering And Graduate School Of Engineering Mie
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