Mechanism of Isoprenaline-Stimulated Diacylglycerol Formation in Rat Parotid Acinar Cells.

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概要

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• The kinetics and mechanism of <I>sn</I>-1, 2-diacylglycerol (DAG) formation induced by isoprenaline were studied in rat parotid acinar cells. DAG accumulation induced by 100 μM isoprenaline reached its maximum at 1 min, rapidly decreased (about 50%) at 5 min and then remained constant for 30 min. DAG accumulation 1 min after isoprenaline treatment was dose-dependent. Either propranolol or phentolamine inhibited isoprenaline-stimulated DAG accumulation in a dose-dependent manner. Addition of a vasoactive intestinal polypeptide, forskolin, or dibutyryl cyclic AMP had no effect on DAG accumulation. Isoprenaline did not cause the release of [<SUP>3</SUP>H]choline or [<SUP>3</SUP>H]ethanolamine metabolites into the medium. Based on the kinetics of DAG formation and [32P]phosphoinositide breakdown, we conclude that isoprenaline-induced DAG formation was mainly related to the hydrolysis of [<SUP>32</SUP>P]phosphatidylinositol 4, 5-bisphosphate ([<SUP>32</SUP>P]PIP<SUB>2</SUB>). These results suggest that the effect of isoprenaline on DAG formation is mediated by α1-adrenoceptor activation, that it is not related to the increase in cyclic AMP, and that it is closely related to PIP<SUB>2</SUB> hydrolysis.

• 公益社団法人 日本薬理学会の論文

著者

• Izawa Tetsuya

  Department Of Exercise And Sport Science Graduate School Of Science Tokyo Metropolitan University

• Suda Kazuhiro

  Department Of Human System Science Graduate School Of Decision Science And Technology Tokyo Institut

• Tsuboi Minoru

  Department Of Pharmacology Tokyo College Of Pharmacy

• Noguchi Masamichi

  Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University

• KOMABAYASHI Takao

  Department of Human System Science, Graduate School of Decision Science and Technology, Tokyo Institute of Technology

• Izawa Tetsuya

  Department of Physiology and Pharmacology, Tokyo College of Pharmacy

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