Discrimination of Maidong (麦冬) Derived from Ophiopogon and Liriope Species by rbcL Sequences, and Their Chemical Components and Tuber Anatomy

概要

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To discriminate the two kinds of Maidong derived from Ophiopogon or Liriope more reliably using genetic information, we sequenced partial rbcL genes of six species of Ophiopogon, three species and one variety of Liriope, and the Maidong from the markets. In this study, six genotypes, five in Ophiopogon and one in Liriope, were recognized. Then, it was possible to discriminate clearly by the rbcL genotype between the Maidong derived from Ophiopogon and Liriope species. We determined the contents of methylophiopogonanone A and B by HPLC that were reported as specific constituents to Maidong of Ophiopogon species. Both methylophiopogonanone A and B were contained only in the Maidong identified as Ophiopogon by the rbcL genotype. On the discrimination of the two kinds of Maidong, methylophiopogonanone A and B are also effective. Additionally, Maidong of Ophiopogon and Liriope, could be identified anatomically with 76% and 93% probability respectively by checking whether or not oil drops in exodermis and velamen occur in the tuber.
日本生薬学会の論文
2004-02-20