Molecular Identification of Tiandong(天冬) Derived from Asparagus cochinchinensis(Lour.) Merrill by Two Typical Deletions in cpDNA
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概要
- 論文の詳細を見る
The swollen and dried roots of Asparagus cochinchinensis (Lour.) Merrill is a crude drug called "Tiandong" (天冬) in China and "Tenmondo" (天門冬) in Japan. It is difficult to identify the botanical origin by roots because the morphological characters are quite simple and some Asparagus species such as A. lycopodineus also have similar swollen roots. To develop an accurate and easy identification procedure of A. cochinchinensis, we evaluated previously reported regions of deletions in chloroplast DNA (cpDNA) of various Asparagus species by the method of PCR amplification. The result indicates that the only A. cochinchinensis has two large deletions. The PCR amplification using samples of A. cochinchinensis from various localities reveals that the deletions are well conserved. Moreover, amplification products were successfully obtained from all market samples and were used for the straightforward identification. From these results, the PCR amplification of the cpDNA regions for Tiandong allows the accurate identification of A. cochinchinensis.
- 日本生薬学会の論文
- 2005-04-20
著者
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FUKUDA Tatsuya
JST Innovation Satellite Kochi
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亀谷 壽昭
東北大学院生命科学研究科
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油田 正樹
Medicinal Evaluation Laboratory Tsumura Research Institute
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Aburada M
Tsumura Research Inst. Pharmacology Ibaraki Jpn
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TAKEDA Shuichi
Pharmacology Research Department, Central Research Laboratories, R & D Division, Tsumura & Co.
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ABURADA Masaki
Pharmacology Research Department, Central Research Laboratories, R & D Division, Tsumura & Co.
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YAMAJI Hiroki
Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura &
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FUKUDA Tatsuya
Graduate School of Life Sciences, Tohoku University
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NAKAMURA Toru
Graduate School of Life Sciences, Tohoku University
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NAKADA Mutsumi
Graduate School of Life Sciences, Tohoku University
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KANNO Akira
Graduate School of Life Sciences, Tohoku University
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Fukuda Tatsuya
Forestry Science Institute Graduate School Of Agriculture Kochi University
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Fukuda Tatsuya
Department Of Ecology And Evolutionary Biology Graduate School Of Life Sciences Tohoku University
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Yokoyama J
Division Of Ecology And Evolutionary Biology Graduate School Of Life Sciences Tohoku University
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竹田 秀一
Tsumura & Co. Tsumura Res. Laboratories
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油田 正樹
ツムラ研究本部 医薬評価研究所
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Yokoyama Jun
Graduate School Of Life Sciences Tohoku University
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Kameya Toshiaki
Graduate School Of Life Sciences Tohoku Univ.
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Song In-ja
Graduate School Of Life Sciences Tohoku University
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Yokoyama J
Department Of Biology Faculty Of Science Yamagata University
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Yamaji Hiroki
Botanical Raw Materials Research Department Tsumura & Co.
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Kanno A
Graduate School Of Life Sciences Tohoku University
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Terabayashi Susumu
Pharmacognosy And Medicinal Resources Research Department Tsumura And Co.
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Terabayashi S
Tsumura Lab. Ibaraki Jpn
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Terabayashi Susumu
Pharmacognosy And Medicinal Resources Research Department Central Research Laboratories Tsumura &
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Terabayashi Susumu
Pharmacognosy Department Tsumura & Co.
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Nakada Mutsumi
Graduate School Of Life Sciences Tohoku University
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Nakamura Toru
Graduate School Of Life Sciences Tohoku University
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Nakamura Toru
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nakamura Toru
Graduate School Of Life And Environment Sciences University Of Tsukuba
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Kanno Akira
Graduate School Of Life Sciences Tohoku University
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Yokoyama Jun
Graduate School Of Law Hitotsubashi University
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Kanno Akira
Graduate School Of Life Sciences Tohoku Univ.
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FUKUDA Tatsuya
Graduate School of Integrated Arts and Sciences, Kochi University
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FUKUDA Tatsuya
Graduate School of Information Science and Technology, Osaka University
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