Species Identification of Licorice Using nrDNA and cpDNA Genetic Markers(Pharmacognosy)

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概要

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• For the accurate identification of medicinal licorice species, nucleotide sequences of four types of DNA regions were researched for 205 specimens, including three species used as licorice: Gfycyrrhiza uralensis, Glycyrrhiza glabra, and Gfycyrrhiza inflata. The four DNA regions were the internal transcribed spacer (ITS) on nuclear ribosomal DNA, the rbcL gene, the matK gene, and the trnH-psbA intergenic region on chloroplast DNA (cpDNA). Ten genotypes were consequently recognized as combinations of the sequence data obtained from the four DNA regions. Species-specific genotypes were defined from the frequency of the appearance of species in each genotype and from the phylogenetic relationships of the 10 genotypes. This revealed the possibility of identifying licorice species based on the 10 genotypes. Next, comparison of species identifications by each DNA region suggested that efficient identification of licorice species is possible using the genetic information obtained from the ITS and trnH-psbA intergenic region. Additionally, concerning the phylogenetic relationships of the Gfycyrrhiza species used as licorice, it is suggested from the genetic information of the four types of DNA regions that G. glabra is more closely related to G. inflata than to G. uralensis. In the G. uralensis examined, four genotypes were recognized as intra specific variations. The appearance frequency of each genotype in G. uralensis differed according to the area in China. G. uralensis may have expanded its distribution areas from western to eastern China because many licorices with the phylogenetic ancestral genotype were observed in western areas, while many with the derivative genotype were observed in eastern areas.

• 公益社団法人日本薬学会の論文
• 2007-08-01

著者

• Kondo Kenji

  Botanical Raw Materials Research Dept. Tsumura & Co.

• Morota Takashi

  Botanical Raw Materials Research Department Tsumura & Co.

• Morota Takashi

  Botanical Raw Materials Research Dept. Tsumura & Co.

• SHOYAMA Yukihiro

  Graduate School of Pharmaceutical Sciences, Kyushu University

• SHIBA Mao

  Botanical Raw Materials Research Department, Tsumura & Co.

• YAMAJI Hiroki

  Botanical Raw Materials Research Department, Tsumura & Co.

• ZHENGMIN Cheng

  Xinjiang Research Institute of Ecology and Geography, Chinese Academy of Science

• HUIXIA Pan

  Xinjiang Research Institute of Ecology and Geography, Chinese Academy of Science

• SHOYAMA Yukihiro

  Kyushu University, Graduate School of Pharmaceutical Sciences

• Morota Takashi

  Pharmacognosy And Medicinal Resources Research Department Central Research Laboratories Tsumura &

• Huixia Pan

  Xinjiang Research Institute Of Ecology And Geography Chinese Academy Of Science

• Zhengmin Cheng

  Xinjiang Research Institute Of Ecology And Geography Chinese Academy Of Science

• Yamaji Hiroki

  Botanical Raw Materials Research Department Tsumura & Co.

• Yamaji Hiroki

  Botanical Raw Materials Division Botanical Raw Materials Research Department Tsumura & Co.

• Shoyama Yukihiro

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Shiba Mao

  Botanical Raw Materials Research Dept. Tsumura & Co.

• Kondo Kenji

  Pharmacognosy Department Tsumura & Co.

• Morota Takashi

  Botanical Raw Materials Research Department Botanical Raw Materials Division Tsumura & Co.

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