Western Blotting Method for the Immunostaining Detection of Glucuronides of Glycyrrhetic Acid Using Anti-Glycyrrhizin Monoclonal Antibody
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概要
- 論文の詳細を見る
A method for detecting glucuronides of glycyrrhetic acid using Western blotting was investigated. Glucuronides of glycyrrhetic acid separated by silica gel TLC were transferred to a polyvinyliden difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a glucuronides of glycyrrhetic acid-BSA conjugate. Individual spots were stained by monoclonal antibody against glycyrrhizin. Immunostaining of glucuronides of glycyrrhetic acid was more sensitive compared to other staining methods. The newly established immunostaining method can be expanded to the distribution of glucuronides of glycyrrhetic acid in the plant body.
- 公益社団法人日本薬学会の論文
- 1999-02-15
著者
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SHOYAMA Yukihiro
Graduate School of Pharmaceutical Sciences, Kyushu University
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Shoyama Yukihiro
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University
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Tanaka Hiroyuki
Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences
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Shan Shao-jie
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Kyushu University
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Shoyama Yukihiro
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyushu University
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Shoyama Yukihiro
Graduate School Of Pharmaceutical Sciences Kyushu University
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TANAKA Hiroryuki
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University
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Tanaka Hiroryuki
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Kyushu University
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Shoyama Yukihiro
Department Of Forestry Faculty Of Agriculture Kyushu University
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Tanaka Hiroyuki
Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic
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SHOYAMA Yukihiro
Department of Chemo-Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kyushu University
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