Pharmacologically Active Saikosaponin in Bupleurum falcatum Detected by Competitive ELISA and Eastern Blotting Using Monoclonal Antibodies(Crop Physiology & Ecology)

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概要

• 論文の詳細を見る

• This study showed the effective microanalytic methods for detection of saikosaponin by a competitive enzyme-linked immunosorbent assay (ELISA) using anti-saikosaponin a (SSa) monoclonal antibody (MAb). The results showed that the competitive ELISA had higher sensitivity than high performance liquid chromatography (HPLC) for the detection of saikosaponin. ELISA showed a highly significant difference in SSa concentrations between the roots of Bupkurum falcatum with different origins. In addition, a difference in SSa concentrations was observed between cytologically different cultivars originating from Japan (2n=26) and Korea (2n=20). It also showed excellent performance in quantitative analysis of SSa in the small samples from regenerated plantlets and calli. In addition, Eastern blotting using anti-SSa MAb 3G10 multispecific to saikosaponins was applied to analyze the distribution of saikosaponins in Bupleurum falcatum roots and leaves. The localized distribution of saikosaponins in whole leaves and cork layer of the roots in B. falcatum was successfully demonstrated by this novel immunoassay.

• 日本作物学会の論文

著者

• Tanaka Hiroyuki

  Department Of Cardiology Kurashiki Central Hospital

• Lee Sang

  College Of Industrial Science Chung-ang University

• Zhu Shu-hang

  Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyushu University

• Tanaka Hiroyuki

  Graduate School of Pharmaceutical Sciences, Kyushu University

• Zhu Shu-hang

  九州大学 大学院薬学府生薬学

• Shon Tae

  R&d Center Istech Inc. Cu Techno Center Catholic University Of Daegu

• Shoyama Yukihiro

  Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University

• Shon Tae

  R&D Center, ISTECH, Inc., CU Techno Center, Catholic University of Daegu

• Tanaka H

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Lee Sang

  College Of Agriculture And Life Sciences Kyungpook National University

• Tanaka Hiroyuki

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Shoyama Y

  Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Nagasaki International University

• Shoyama Yukihiro

  Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyushu University

• Tsukikawa Hiroshi

  Department Of Pharmacognosy Graduate School Of Medical Science

• Shoyama Yukihiro

  Department Of Forestry Faculty Of Agriculture Kyushu University

• Tanaka H

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Tanaka Hiroyuki

  Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic

• SHOYAMA Yukihiro

  Department of Chemo-Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kyushu University

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