A Quantitative ELISA Using Monoclonal Antibody to Survey Paeoniflorin and Albiflorin in Crude Drugs and Traditional Chinese Herbal Medicines
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概要
- 論文の詳細を見る
This work describes an immunochemical approach for the quality control of Paeoniae Radix by an enzyme-linked immunosorbent assay (ELISA) based on determination of the total concentration of paeoniflorin (PF) and albiflorin (Alb), which are major bioactive constituents in Paeoniae Radix. Four hybridromas secreting monoclonal antibodies against PF and Alb were produced by fusing splenocytes from a mouse immunized by a PF-bovine serum albumin (BSA) conjugate with the hypoxanthine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A relatively higher reactivity of monoclonal antibodies (MAbs) with PF and Alb than oxypaeoniflorin (OP) and benzoylpaeoniflorin (BP) was observed, while other monoterpenes and benzoic acid did not cross-react. When PF was used as a standard, the assay can cover a measuring range of 20-600ng/ml for PF and Alb. A series of Paeoniae Radix samples have been determined, and the results showed good agreement with that determined by traditional high-performance liquid chromatography (HPLC). The developed competitive ELISA was 100 times more sensitive than the HPLC method. Meanwhile, fifteen Chinese traditional prescriptions were determined by the competitive ELISA
- 公益社団法人日本薬学会の論文
- 2003-06-01
著者
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Tanaka Hiroyuki
Department Of Cardiology Kurashiki Central Hospital
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Morinaga Osamu
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Nagasaki International University
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Morinaga Osamu
九州大学 薬学府薬用植物育種学
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Morinaga Osamu
Department Of Internal Medicine Aiseikai Yamashina Hospital
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Tanaka Hiroyuki
Graduate School of Pharmaceutical Sciences, Kyushu University
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Tanaka H
Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences
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Tanaka Hiroyuki
Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences
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Shoyama Y
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Nagasaki International University
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Shoyama Yukihiro
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyushu University
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Tsukikawa Hiroshi
Department Of Pharmacognosy Graduate School Of Medical Science
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LU Zhaohua
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University
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Shoyama Yukihiro
Department Of Forestry Faculty Of Agriculture Kyushu University
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Tanaka H
Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences
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Tanaka Hiroyuki
Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic
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Lu Zhaohua
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Kyushu University
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SHOYAMA Yukihiro
Department of Chemo-Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kyushu University
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