Formation of a Monoclonal Antibody against Glycyrrhizin and Development of an ELISA
スポンサーリンク
概要
- 論文の詳細を見る
The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3-X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml^<-1> to 200 ng ml^<-1> of glycyrrhizin.
- 公益社団法人日本薬学会の論文
- 1998-12-15
著者
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Tanaka Hiroyuki
Department Of Cardiology Kurashiki Central Hospital
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Shoyama Yukihiro
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Kyushu University
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Shoyama Yukihiro
Department Of Forestry Faculty Of Agriculture Kyushu University
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Tanaka Hiroyuki
Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic
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Tanaka Hiroyuki
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Kyushu University
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Syoyama Yukihiro
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University
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