Double Staining of Ginsenosides by Western Blotting Using Anti-ginsenoside Rb1 and Rg1 Monoclonal Antibodies
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概要
- 論文の詳細を見る
Ginsenosides separated by silica gel TLC blotted to a polyvinylidene difluoride(PVDF)membrane was treated with a NalO_4 solution followed by bovine serum albumin(BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with anti-ginsenoside Rg1 and Rb1 monoclonal anti-bodies(MAbs). The newly established double staining with the Western blotting methods was applied for the determination of ginsenosides possessing protopanaxadiol or protopanaxatriol and the number of sugar depending on the stained color and their Rf values in the Panax plants.
- 公益社団法人日本薬学会の論文
- 2001-10-01
著者
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Fukuda N
Graduate School Of Pharmaceutical Sciences Kyushu University
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Tanaka Hiroyuki
Graduate School of Pharmaceutical Sciences, Kyushu University
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Fukuda Noriko
Graduate School Of Pharmaceutical Sciences Kyushu University
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SHOYAMA Yukihiro
Graduate School of Pharmaceutical Sciences, Kyushu University
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Tanaka H
Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences
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Tanaka Hiroyuki
Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences
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Shoyama Y
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Nagasaki International University
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Shoyama Yukihiro
Graduate School Of Pharmaceutical Sciences Kyushu University
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Tanaka Hiroyuki
Graduate School Of Pharmaceutical Sciences Kyushu University
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