Acute Regulation by Dietary Phosphate of the Sodium-Dependent Phosphate Transporter (NaPi-2) in Rat Kidney.
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概要
- 論文の詳細を見る
Alteration of the dietary intake of phosphate (Pi) leads to rapid changes in renal Pi transport activity. The present study, examined the underlying cellular mechanisms of the rapid regulation, with special reference to renal P, cotransporter. Rats were fed either a low-Pi (0. 02%) diet (CLP rats), the low-Pi diet followed by a high-P, (1. 2%) diet (AHP rats), or a normal (0. 6%) diet (control rats). Nat-dependent Pi transport activity in the brush border membrane was significantly increased in CLP rats compared with control rats, and this activity decreased rapidly within 2 h after the change of diet in AHP rats. Kinetic analysis of Pi transport in the AHP rats indicated that the reduction was accompanied by a decrease in the apparent Vmax, for Na+-dependent Pi uptake. Northern blot analysis showed no difference in the abundance of NaPi-2 mRNA of the kidney between AHP and CLP rats. In contrast, Western blot analysis of renal brush border membrane proteins of AHP rats indicated a significant decrease in the abundance of NaPi-2 protein as compared with CLP rats. Immunoreactive signals for NaPi-2 were detected in lysosomal fractions of AHP and CLP rats. Immunohistochemical analysis showed that, NaPi-2 immunoreactivity in AHP rats was largely reduced in the apical membrane of the proximal tubular epithelial cells. Neither cycloheximide nor actinomycin D affected high-Pi-induced reduction of NaPi-2 protein in the brush border membrane of AHP rats, indicating that de novo protein synthesis of an unidentified regulator protein was not involved in the mechanism of this reduction. In contrast, treatment with colchicine, which disrupts microtubulers, abolished the effect of high-Pi diet on NaP, -2 expression. These results suggested that rapid en-docytotic internalization of NaP, -2 may occur specificallyin the brush border membrane following an acute increase in dietary Pi intake.
- 社団法人 日本生化学会の論文
著者
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Fukui Yoshihiro
Department Of Anatomy And Developmental Neurobiology Institute Of Health Biosciences The University
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Haga Hiromi
Department Of Anatomy And Developmental Neurobiology University Of Tokushima School Of Medicine
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Arai Hidekazu
Department Of Applied Chemistry Faculty Of Engineering Toyama University
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Tatsumi Sawako
Department Of Molecular Nutrition Institute Of Health Biosciences The University Of Tokushima Gradua
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Segawa Hiroko
Department Of Molecular Nutrition Institute Of Health Bioscience University Of Tokushima Graduate Sc
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Morita Kyoko
Department Of Biology Faculty Of Science Kumamoto University
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Katai Kanako
Department Of Clinical Nutrition School Of Medicine Tokushima University
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Taketani Yutaka
Department Of Clinical Nutrition Institute Of Health Biosciences The University Of Tokushima
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Miyamoto Ken-ichi
Department Of Applied Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
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Hisano Setsuji
Department Of Anatomy School Of Medicine Tokushima University
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Takeda Eiji
Department Of Applied Physics Faculty Of Engineering Osaka University
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Segawa Hiroko
Departments of Clinical Nutrition, School of Medicine, Tokushima University
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