Purification and Kinetic Properties of Butyryl-CoA Synthetase from Paecilomyces varioti(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
A butyryl-CoA synthetase (EC 6.2.1.2) was obtained from parecilomyces varioti AHU 9417 in a highly purified form, which appeared to be homogeneous on polyacrylamide gel electrophoresis in the absence and presence of sodium dodecyl sulfate (SDS). The purified enzyme exhibited molocular weights of 145,000 for the native enzyme and 74,000 for the subunit. This enzyme required both monovalent and divalent cations for the reaction. The enzyme was active toward saturated fatty acids with 3 to 5 carbon atoms, and butyrate was the best substrate. The reaction mechanism of the enzyme was also investigated and was found to conform to a Bi-Uni-Uni-Bi ping-pong mechanism, in which the order of addition and evolution of substrates and products was ATP, butyrate, PPi, CoA, butyryl-CoA (or AMP) and AMP (or butyryl-CoA). Butyryl-CoA and AMP were evolved at random or at the same time.
- 社団法人日本農芸化学会の論文
- 1987-01-23
著者
-
Ito Takashi
Department Of Chemistry School Of Science The University Of Tokyo
-
Takao Shoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
-
Tanida Masatoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
-
ITO Takashi
Department of Advanced Materials Chemistry, Graduate School of Engineering, Yokohama National University
関連論文
- Proteome approach for identification of schistosomiasis japonica vaccine candidate antigen.
- Newly identifi ed message for rescue and repair from necrotic cell: Biology and clinical relevance of“endokine, HMGB1”
- Seasonal Variation of Trace Element Loss to Sweat during Exercise in Males
- Concentrations of Trace Elements in Sweat during Sauna Bathing
- Temperature-Dependent Behavior of Impurity Atoms Implanted in Highly Oriented Pyrolytic Graphite : An Application of a New Online TDPAC Method
- Huge Cystic Lymphangioma of the Pancreas : Report of a Case
- Nanoscale ErP Islands on InP(001) Substrate Grown by Organometallic Vapor-Phase Epitaxy
- Thermal Quenching of Er-Related Luminescence in GaInP Doped with Er by Organometallic Vapor Phase Epitaxy
- Effects of Growth Temperature on Er-Related Photoluminescence in Er-Doped InP and GaAs Grown by Organometallic Vapor Phase Epitaxy with Tertiarybutylphosphine and Tertiarybutylarsine
- Potentiometric Responses of Ionophore-Incorporated Bilayer Lipid Membranes with and without Added Anionic Sites
- Oxidative Addition Reactions Involving Molybdenum Polyhydrides
- NMR Structure of the Heterodimer of Bem1 and Cdc24 PB1 Domains from Saccharomyces Cerevisiae
- Synthesis of Novel Amidinato(amidine) Complexes of Molybdenum via Bis(amidine) Complexes
- Synthesis and Crystal Structure of N-Heterocyclic Carbene Complexes of Bis(η^5-cyclopentadienyl)molybdenum
- Molybdenum Hydrido Complex Containing a Novel Quinquidentate (P,P,Ge,P,P) Ligand
- Dimer Formation of Anthracene in Langmuir-Blodgett Films as Revealed by Time-Resolved Fluorescence Spectra
- Evolution of Local Magnetic State in SmRu_4P_ Probed by Muon Spin Relaxation(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Videomanometry of the pelvic organs : A comparison of the normal lower urinary and gastrointestinal tracts
- Nocturnal Polyuria with Abnormal Circadian Rhythm of Plasma Arginine Vasopressin in Post-stroke Patients
- Tryptophan Production by a Lipoic Acid Auxotroph of Enterobacter aerogenes Having Both Pyruvic Acid Productivity and High Tryptophanase Activity(Microbiology & Fermentation Industry)
- A Systematic Study on the Complexation of Quaternary Ammonium Salts and Neutral Phenols
- A variety of lipophilic amines incorporated in liquid membranes exhibit potentiometric responses to neutral phenols
- Syntheses, Structure, and Some Reactions of Di-μ-Hydroxo Dinuclear Complexes of Tungsten(IV) and Molybdenum(IV)
- Preparation and Some Reactions of the Tungsten Hydridotosylato Complex Cp_2 WH(OT_s)
- Distal Gastrectomy with Reconstruction of the Right Gastroepiploic Artery for Gastric Cancer After Coronary Artery Bypass Grafting : Report of a Case
- Effects of Topically Applied Capsaicin Cream on Neurogenic Inflammation and Thermal Sensitivity in Rats
- Serum α 1-Antichimotrypsin Levels and Cardiovasucular Risk Factors in the Japanese Elderly Population
- Novel Microstructures of Self-Assembled Microcrystallites of (NH_4)_3PW_O_ and Cs_3PW_O_
- Porous Aggregates of Unidirectionally Oriented(NH_4)_3PW_O_Microcrystallites : Epitaxial Self-Assembly
- Surgical Repair for Tracheo-Innominate Artery Fistula with a Muscle Flap
- Successful Surgical Management of Patients With Infective Endocarditis Associated With Acute Neurologic Deficits
- Prenatal diagnosis of congenital myotonic dystrophy in two Japanese families: direct mutation analysis by a non-radioisotope PCR method and haplotype analysis with flanking DNA markers
- Changes in Sleep Apnea Before and After Atrial Defibrillation : Is Sleep Apnea Improved by Rhythm Control?(Arrhythmia, Non-pharmacological Therapy 11 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Reduction of Imines to Amines through Use of the Cp_2MoH_2 and Protonic Acid System
- Autonomic function in the early stage of panic disorder : Power spectral analysis of heart rate variability
- Phenobarbital Following Phototherapy for Crigler-Najjar Syndrome Type II with Good Fetal Outcome : A Case Report
- Repetitive intermittent hypoxia-ischemia and brain damage in neonatal rats
- Placenta Previa Increta Penetrating the Entire Thickness of the Uterine Myometrium : Ultrasonographic and Magnetic Resonance Imaging Findings
- On the Determination of Microwave Conductivity from Eddy Current Loss in Planar Specimens of High Conductivities
- ESR Studies on Thermal Hysteresis of MnSe
- Magnetic Susceptibility, Thermal Expansion and Electrical Resistivity of MnSe
- Identification of connexins in human oral mucosa and therapeutic effect of irsogladine maleate on aphthous stomatitis
- Two-Dimensional Electrophoresis/Phage Panning (2D-PP): A Novel Technology for Direct Antibody Selection on 2-D Blots
- Medulla and Gut
- Cellulase Induction by SOluble Acetyl Cellobioses in Penicillium purpurogenum
- Dissociation of MeV HeH^+ Ions at Glancing Angle Incidence on Clean (001) Surface of SnTe
- Anomalous g-Anisotropy in the ESR Signals of Halogen-Doped AlPcF Thin Films
- Antiferromagnetism of GaPcF・(DDQ)_x
- Occurrence of Antiferromagnetic Transition in Iodine Doped AlPcF Film
- Ultrasonic Investigation of Double Ferromagnetism in Gd_Y_Sc_ Single Crystal Alloys
- Ultrasonic Investigation on Gd_Sc_ Single-Crystal Alloy with Antiphase Domain Structure
- An Electron Spin Resonance Study of AIPcF・(DDQ)_x Film
- A Pressure Dependent Antiferromagnetism of AlPcF・(TCNE)_xFilm
- Antiphase Domain Structure in Gd_Sc_ Single-Crystal Alloys
- Ultrasonic Study of a Single Crystal Gd_Y_Lu_ Alloy in a Magnetic Field
- Synthesis and Characterization of Novel Phosphido-Bridged Bimetallic Complexes of Molybdenum and Tungsten
- Analysis of Bacterial Flora in Dohyo Soil
- Purification and Some Properties of Isocitrate Lyase from Paecilomyces varioti with High Malic Acid Production
- Purification and Characteristics of Two Exoinulinases from Chrysosporium pannorum
- Inulinase from Chrysosporium pannorum
- The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica
- Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic Yeast Pichia methanolica
- Syntheses and Spectroscopic Properties of Substituted Phthalimido Complexes of Molybdenum(II)
- Synthesis and Some Reactions of Molybdenocene Derivatives Coordinated with the Chiral Carboxylato Ligands
- Reactions of Molybdenocene and Tungstenocene Derivatives with Cobalt Carbonyls and the Related Compounds
- Purification and Some Properties of Two Cellobiohydrolases from Penicillium purpurogenum
- Synthesis and Structural Characterization of the New Dioxygen Complexes of Molybdenum(IV)
- Degradation of Dimethyl Disulfide by Pseudomonas fluorescens Strain 76
- Tissue characterization of uterine myometrium using the ultrasound gray-level histogram width
- Admittance Study of a Pt/C_/In/A1 Schottky-Barrier Cell
- Electrically Detected Electron Spin Resonance of Pt/C_/In/Al Schottky-Junction System
- Purification and Substrate Specificity of α-Glucosidase from Paecilomyces varioti AHU 9417
- Degradation of Tough Materials by Cellulase from Corticium rolfsii(Microbiology & Fermentation Industry)
- Purification and Properties of Two Endocellulases from Penicillium purpurogenum(Biological Chemistry)
- Production of a Raw Starch Saccharifying Enzyme by Corticium rolfsii(Microbiology & Fermentation Industry)
- Screening of Microorganisms for Raw Starch Saccharifying Enzyme Production
- Cellulase Production by Penicillium purpurogenum
- ACUTE AND SUBACUTE TOXICITY STUDIES ON COLLAGEN WOUND DRESSING (CAS) IN MICE AND RATS
- Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Novel Concurrent Formation of Mo-Si and Si-C Bonds by the Reaction between [MoH_4(Ph_2PCH_2CH_2PPh_2)_2] and PhSiH_3
- Reactions of Alkyl malonates with Molybdenum and Tungsten Complexes. Syntheses and Structure Hydrido-Malonato Complexes
- Electron Microscopic Studies on Bactericidal Effects of Electrolyzed Acidic Water on Bacteria Derived from Kendo Protective Equipment
- Indication for Histological Examination of Endometrium in Breast Carcinoma Patients Receiving Tamoxifen Therapy
- Fetal Ascites Immediately after Rupture of Ovarian Cysts
- Scanning Tunneling Microscopy of UV-Induced Gasification Reaction on Highly Oriented Pyrolytic Graphite
- Flow Field and Performance Analysis of an Annular-Type Aerospike Nozzle with Base Bleeding
- Three-Dimensional Antiferromagnetic Transition of Brominated Fluoro-Aluminum Phthalocyanine Polymer
- Building of Observers to Detect Faults for Batch Processes Based on Discrete Event Systems Approaches
- Integration between Scheduling and Design of Batch Systems Based on Petri Net Models
- Purification and Kinetic Properties of Butyryl-CoA Synthetase from Paecilomyces varioti(Microbiology & Fermentation Industry)
- Quasi-arithmetic means of continuous functions
- Dielectric Properties of Multilayered Ferroelectric Thin Films Fabricated by Sol-Gel Method ( FERROELECTRIC MATERIALS AND THEIR APPLICATIONS)
- Synthesis of Iron(III) Complex Bearing Tridentate β-Aminoketonato Ligand : Application to Iron-catalyzed Cross-coupling Reaction of Arylmagnesium Bromides with Alkyl Halides
- C-12-16 A 2-Stage Analog to Digital Converter Combining Successive Approximation and Integrating Techniques
- JUXTAGLOMERULAR CELLS IN MICE AFTER LONG-TERM TREATMENT WITH CAPTOPRIL : An Electron Microscopic Study
- Influence of resin coating materials on Porphyromonas gingivalis attachment
- Improvement of ArF Photo Resist Pattern by VUV Cure(Lithography-Related Techniques,Fundamentals and Applications of Advanced Semiconductor Devices)
- Selective Determination of Trace Iron in Different Oxidation States in Natural Water by Flow Injection-Solid Phase Spectrometry
- Stable Readout Circuit for Ferroelectric Random Access Memory Using Complementary Metal–Oxide–Semiconductor-Inverter-Based Capacitive-Feedback Charge-Integration Scheme
- Above-Complementary Metal–Oxide–Semiconductor Inductor for Rapid Prototyping of Mixed-Signal System on Chips