Purification and Some Properties of Endoinulinase form Chrysosporium Pannorum
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概要
- 論文の詳細を見る
One endoinulinase (2,1-β-D-fructan fructanohydrolase EC 3.2.1.7) was purified from Chrysosporium pannorum AHU 9700. The enzyme was a glycoprotein having an isoelectric point around pH 3.8. The molecular weight was 58,000 and 56,000 by SDS-polyacrylamide gel electrophoresis and gel filtration on Sephacryl S-200,respectively. The endoinulinase was most active between pH 6.0-7.0 at 50℃, and was stable at 45℃ (10 min) and from pH 4.5 to 8.5 (24h). This enzyme was active only on inulin, not on levan, sucrose, raffinose, or melezitose. The main products from inulin ware inulotriose, inulotetraose, and inulopentaose.
- 社団法人日本生物工学会の論文
- 1989-04-25
著者
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Takao S
Hokkaido Univ. Sapporo Jpn
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TANIDA MASATOSHI
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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Xiao Rong
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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TANIDA MASATOSHI
(Present address)Hokkaido Green-Bio Institute
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Takao S
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Tanida M
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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