Degradation of Benzoate by an Anaerobic Consortium and Some Properties of a Hydrogenotrophic Methanogen and Sulfate-Reducing Bacterium in the Consortium

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A highly simplified anaerobic consortium which was able to degrade benzoate under mesophilic conditions was obtained from digested sludge acclimatized with benzoate. It converted 5 mM benzoate to methane quantitatively within 3 weeks in the absence of any organic nutrients under an N_2/CO_2 atmosphere. Degradation of benzoate was strictly inhibited by hydrogen. The consortium consisted of at least three microorganisms including an autofluorescent irregular coccus which was identified as Methanogenium sp., a short rod which did not autofluoresce and was considered to be a benzoate degrader, and a filamentous bacterium apparently classified as Methanothrix (="Methanosaeta"). When sulfate was added to the medium, the methanogens were readily replaced by a sulfate-reducing bacterium, probadly belonging to the genus Desulfovibrio, which had still remained in very low number in the consortium in the absence of sulfate, and benzoate was stoichiometrically converted to acetate witout methanogenesis. Of various compounds which were expected to be intermediates in the benzoate degradation, only crotonate was degraded by concentrated cells of the consortium.
社団法人日本生物工学会の論文
1992-03-25