Sensitive Detection of LOH Events in a Human Cell Line after C-ion Beam Exposure

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概要

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• A molecular analysis of the loss of heterozygosity (LOH) events in human cells after low-dose heavy-ion exposure could contribute to the sensitive detection of the genetic influences caused by high-LET radiation. We exposed human lymphoblastoid TK6-20C cells to 10 cGy of an accelerated C-ion (22 keV/μm) beam, and observed a 3.1-fold increase in the mutation frequency (MF) at the heterozygous thymidine kinase (TK) locus over the background level. This increase was due to the induction of TK mutants exhibiting hemizygous-type LOH. Surprisingly, the frequency of type-2 hemizygous LOHs (interstitial deletions) was about 23-fold, induced over the background level, and the LOH extent patterns of this type 2 induced after the irradiation were clearly different from that of the spontaneous background. . Since hemizygous-type LOH mutants are considered to be the result of the end-joining repair of DNA double-strand breaks (DSB), C-ions may more efficiently induce DSBs than X-rays in this low-dose region. In addition, an enhanced misrepair of C-ion-induced DSBs might also account for the induction of radiation-specific hemizygous-type LOH.

• 日本放射線影響学会の論文

著者

• HONMA Masamitsu

  Natl. Inst. Health Sci.

• HONMA Masamitsu

  Division of Genetics and Mutagenesis, National Institute of Health Sciences

• YATAGAI Fumio

  IPCR

• Yatagai F

  Riken Saitama Jpn

• Yatagai Fumio

  Division Of Radioisotope Technology Riken

• MORIMOTO Shigeko

  Div. Radioisotope Technology RIKEN

• MORIMOTO Shigeko

  RIKEN

• MORIMOTO SHIGEKO

  Division of Radioisotope Technology, The Institute of Physical and Chemical Research (RIKEN)

• HONMA Masamitu

  Genetic and mutagenesis NIHS

• Honma M

  Division Of Genetics And Mutagenesis National Institute Of Health Sciences

• Honma Masamitsu

  Division Of Genetics And Mutagenesis National Institute Of Health Sciences

• Yatagai F

  Riken Institute

• Honma Masamitsu

  Division Of Genetics And Mutagenesis National Institute Of Health Science

• Morimoto Shigeko

  Division of Radioisotope Technology

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