Extension of In vitro Life-span of γ-irradiated Human Embryo Cells Accompanied by Chromosome Instability

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概要

• 論文の詳細を見る

• We studied the effect of repeated irradiation with a low dose rate (about 0.0012 cGy per mm) of ^<60> Co γ-rays on the in vitro life-span of human embryo (HE) cells. HE cells were cultured in an incubator that was set in a ^<60>Co γ-ray-irradiation room, and the irradiation was repeated throughout the life-span of the HE cells (for about 150 to 160 days) on every day base. During this period, the cells accumulated 106 to 123 cGy. The life-span of the irradiated cells prolonged 1.14 to 1.35 times when compared to that of non-irradiated cells. The incidence of cells with chromosome bridge and micronuclei significantly increased in the irradiated cells. Although the number of chromosomes gradually changed with repetition of culture in both non-irradiated and irradiated cells, the frequencies of aneuploid cells in irradiated cells were about two times higher than that in non-irradiated cells. These results indicate that repeated irradiation with a low dose of γ-rays produces chromosome instability, and it may be a cause of numerical chromosome abnormalities and life-span extension of irradiated cells.

• 日本放射線影響学会の論文

著者

• Suzuki Minoru

  Particle Radiation Oncology Research Center Kyoto University

• NAGATA Kenji

  Particle Radiation Oncology Research Centre, Research Reactor Institute, Kyoto University

• YATAGAI Fumio

  IPCR

• KODAMA SEIJI

  Laboratory of Radiation and Life Science, Department of Health Sciences, School of Pharmaceutical Sc

• WATANABE Masami

  Laboratory of Radiation Biology, Division of Radiation Life Science, Department of Radiation Life Sc

• Suzuki Masao

  National Institute of Radiological Science

• Kodama Seiji

  Division Of Radiation Biology And Health Science Research Center For Radiation And Radioisotopes Res

• YATAGAI Fumio

  The Institute of Physical and Chemical Research

• Yatagai F

  Riken Saitama Jpn

• Yatagai Fumio

  Division Of Radioisotope Technology Riken

• Yatagai Fumio

  Inst. Phys. Chem. Res.

• Yatagai Fumio

  Division Of Radioisotope Technology The Institute Of Physical And Chemical Research

• Yatagai Fumio

  The Inst. Of Physical And Chemical Research

• Yatagai Fumio

  Ri Inst. Phys. Chem. Res.

• YANG ZHI

  Laboratory of Radiation and Life Science, Department of Health Science, School of Pharmaceutical Sci

• NAKANO KAZUSHIRO

  The Institute of Physical and Chemical Research

• SUZUKI KEIJI

  Laboratory of Radiation and Life Science, Department of Health Science, School of Pharmaceutical Sci

• Suzuki Masao

  National Inst. Radiological Sci. Chiba Jpn

• Suzuki Masao

  Heavy-ion Radiobiology Res. Group National Inst. Radiological Sciences

• Yatagai F

  Division Of Radioisotope Technology Riken

• Watanabe M

  Nagasaki University

• Watanabe Masami

  Laboratory Of Plant Nutrition Faculty Of Horticulture Chiba University

• Suzuki K

  Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive

• Yang Z

  Laboratory Of Radiation And Life Science Department Of Health Sciences School Of Pharmaceutical Scie

• Yatagai Fumio

  理化学研究所

• Watanabe Masani

  Division Of Radiation Biology Faculty Of Pharmacology Of Science Nagasaki University

• Suzuki K

  Division Of Radiation Biology Department Of Radiology And Radiation Biology Course Of Life Sciences

• Yang Zhi

  Laboratory Of Radiation And Life Science Department Of Health Sciences School Of Pharmaceutical Scie

• Yatagai F

  Riken Institute

• Kodama Seiji

  Laboratory Of Radiation And Life Science Department Of Health Sciences School Of Pharmaceutical Sciences Nagasaki University

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