Radiation-quality Dependent Cellular Response in Mutation Induction in Normal Human Cells
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概要
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We studied cellular responses in normal human fibroblasts induced with low-dose (rate) or low-fluence irradiations of different radiation types, such as gamma rays, neutrons and high linear energy transfer (LET) heavy ions. The cells were pretreated with low-dose (rate) or low-fluence irradiations (~1 mGy/7-8 h) of 137Cs gamma rays, 241Am-Be neutrons, helium, carbon and iron ions before irradiations with an X-ray challenging dose (1.5 Gy). Helium (LET = 2.3 keV/μm), carbon (LET = 13.3 keV/μm) and iron (LET = 200 keV/μm) ions were produced by the Heavy Ion Medical Accelerator in Chiba (HIMAC), Japan. No difference in cell-killing effect, measured by a colony forming assay, was observed among the pretreatment with different radiation types. In mutation induction, which was detected in the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus to measure 6-thioguanine resistant clones, there was no difference in mutation frequency induced by the X-ray challenging dose between unpretreated and gamma-ray pretreated cells. In the case of the pretreatment of heavy ions, X-ray-induced mutation was around 1.8 times higher in helium-ion pretreated and 4.0 times higher in carbon-ion pretreated cells than in unpretreated cells (X-ray challenging dose alone). However, the mutation frequency in cells pretreated with iron ions was the same level as either unpretreated or gamma-ray pretreated cells. In contrast, it was reduced at 0.15 times in cells pretreated with neutrons when compared to unpretreated cells. The results show that cellular responses caused by the influence of hprt mutation induced in cells pretreated with low-dose-rate or low-fluence irradiations of different radiation types were radiation-quality dependent manner.Translated and modified from Radiat. Biol. Res. Comm. Vol.43(4); 424-435, (2008, in Japanese)
- 日本放射線影響学会の論文
- 2009-09-16
著者
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Suzuki Minoru
Particle Radiation Oncology Research Center Kyoto University
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Suzuki M
Particle Radiation Oncology Research Center Kyoto University
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Seki Masaya
Heavy-ion Radiobiology Research Group Research Center For Charged Particle Therapy National Institut
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Liu Cui
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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SUZUKI Masao
Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology
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Suzuki Masao
National Inst. Radiological Sci. Chiba Jpn
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TSURUOKA Chizuru
Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
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UCHIHORI Yukio
Fundamental Technology Center, National Institute of Radiological Sciences
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KITAMURA Hisashi
Fundamental Technology Center, National Institute of Radiological Sciences
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Suzuki Masao
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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Tsuruoka Chizuru
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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Suzuki Masao
Heavy-ion Radiobiology Research Group Research Center For Charged Particle Therapy National Institut
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Uchihori Yukio
Fundamental Technology Center National Institute Of Radiological Sciences
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Kitamura Hisashi
Fundamental Technol. Center National Inst. Of Radiological Sciences
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Kitamura Hisashi
Fundamental Technology Center National Institute Of Radiological Sciences
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Suzuki Masao
Research & Development Center, Hitachi Chemical Co., Ltd.
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