Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation

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概要

• 論文の詳細を見る

• Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/μm carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing.

• 日本放射線影響学会の論文

著者

• 谷田貝 文夫

  理化学研究所戎崎計算宇宙物理研究室

• WATANABE Masami

  Nagasaki Univ.

• Suzuki Masao

  National Institute of Radiological Science

• Hanaoka Fumio

  理化学研究所放射線生物学研究室

• Kato Takesi

  School Of Medicine Osaka University

• Kato Tomohisa

  関西外国語大学

• Kato Tomohisa

  Radiation Biology Center Kyoto University

• Kase Youko

  National Institute Of Radiological Sciences

• KAGAWA YASUHIRO

  理化学研究所放射線生物学研究室

• KOBAYASHI AKIKO

  Toray Research Center Inc.

• HIRANO MASAHIKO

  Toray Research Center Inc.

• 谷田貝 文夫

  理化学研究所放射線生物学研究室

• 谷田貝 文夫

  理化学研究所

• Suzuki Masao

  National Inst. Radiological Sci. Chiba Jpn

• Kato Tomohisa

  Radiat. Biol. Center Kyoto University

• Kase Yoko

  Space And Particle Radiation Science Research Group National Institute Of Radiological Sciences

• Hirano Masahiko

  Toray Res. Center

• Kase Yoko

  Research Center Of Charged Particle Therapy Natl Inst Radiol Sci.

• Kobayashi A

  Pola Chemical Ind. Inc. Kanagawa Jpn

• Watanabe Masami

  Nagasaki University

• Suzuki Masao

  National Institute Of Radiological Sciences

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