Repair Kinetics of DNA-DSB Induced by X-rays or Carbon Ions under Oxic and Hypoxic Conditions

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概要

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• We studied the relation between initial DNA double-strand breaks (DNA-DSB) and the rejoining kinetics of the strand breaks, as well as the OER (oxygen enhancement ratio) after low- and high-LET (linear energy transfer) radiations. CHO cells were exposed to 200kVp X-rays or 80keV/μm carbon ions under oxic and hypoxic conditions. DNA-DSB in the cells were analyzed by a static-field gel electrophoresis (SFGE). The kinetics of the rejoining could be described by a sum of fast and slow components. The initial released DNA after X-ray irradiation was higher for cells irradiated under an oxic condition than that under a hypoxic condition. The OER of DNA-DSB after X-ray irradiation was 5.7. This value decreased rapidly to be 3.4 with the fast component by 15 minutes. On the other hand, the OER of DNA-DSB after carbon ion irradiation was 2.2, and this value was not changed by rejoining incubation. The OER values for cell killing were 2.8 and 1.8 after X-ray and carbon ion irradiations, respectively. These values matched to the OER for DNA-DSB with complete rejoining. We conclude that the rejoining of DNA-DSB is an important factor in the mechanism of the oxygen effect.

• 日本放射線影響学会の論文
• 2005-09-30

著者

• 安藤 興一

  放医研

• FURUSAWA Yoshiya

  Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Instit

• 安藤 興一

  学振科技特

• 安藤 興一

  Natl. Instit. Rad. Sci. Med.

• FURUSAWA Yoshiya

  National Institute of Radiological Sciences

• FUTAMI Yasuyuki

  Shizuoka Cancer Center

• Futami Yasuyuki

  茨城県立医療大学 放射線技術科学科

• Futami Yasuyuki

  The Institute Of Physics University Of Tsukuba

• Futami Yasuyuki

  Dvisin Of Accelerator Physics And Engineering National Institite Of Radiological Sciences Chiba Japa

• Ando K

  Division Of Health Sciences Graduate School Of Medicine Osaka University

• Ando Koichi

  Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences

• FUKAWA Takeshi

  Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences

• Ando Soichiro

  National Institute Of Radiological Sciences

• Ando Koichi

  Department Of Technical Support And Development Natl. Inst. Radiol. Sci.

• Ando Koichi

  Space And Particle Radiation Science Research Group National Institute Of Radiological Sciences

• Ando Koichi

  Division Of Health Sciences Graduate School Of Medicine Osaka University

• Fukawa Takeshi

  Heavy-ion Radiobiology Research Group National Institute Of Radiological Sciences

• Hirayama Ryoichi

  Heavy-ion Radiobiology Research Group Research Center For Charged Particle Therapy National Institut

• Hirayama Ryoichi

  Heavy-ion Radiobiol. Res. Gr. Natl. Inst. Radiol. Sci.

• Furusawa Y

  Research Center For Charged Particle Therapy National Institute Of Radiological Sciences

• Furusawa Yoshiya

  高エネルギー加速器研究機構物質構造科学研究所

• HIRAYAMA Ryoichi

  Graduate School of Science and Technology, Chiba University

• ANDO Koichi

  Graduate School of Science and Technology, Chiba University

• Fukawa Takeshi

  放射線医学総合研究所

• Hirayama Ryoichi

  Grad. Sch. Sci. Tech. Chiba Univ.

• Furusawa Yoshiya

  Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences

• Ando Koichi

  International Space Radiation Laboratory National Institute Of Radiological Sciences

• Furusawa Yoshiya

  Div. Of Accelerator Res. Natl. Inst. Of Radiol. Sci.

• Furusawa Yoshiya

  Heavy-ion Radiobiol. Res. Gr. Res. Center Charged Particle Therapy Nirs.

• Fukawa Takeshi

  慶応義塾大学

• Ando Koichi

  Graduate School of Life and Environmental Sciences, University of Tsukuba

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