Analysis of Unrejoined Chromosomal Breakage in Human Fibroblast Cells Exposed to Low- and High-LET Radiation
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概要
- 論文の詳細を見る
Reported studies of DNA breakage induced by radiation of various qualities have generally shown a higher fraction of unrejoined residual breaks after high-LET exposure. This observation is supported by the argument that high-LET radiation induced DNA breaks that are more complex in nature and, thus, less likely to be repaired. In most cases the doses used in these studies were very high. We have studied unrejoined chromosome breaks by analyzing chromosome aberrations using a fluorescence in situ hybridization (FISH) technique with a combination of whole chromosome specific probes and probes specific for the telomere region of the chromosomes. Confluent human fibroblast cells (AG1522) were irradiated with γ rays, 490 MeV/nucleon Si, or with Fe ions at either 200 and 500 MeV/nucleon, and were allowed to repair at 37 ℃ for 24 hours after exposure. A chemically induced premature chromosome condensation (PCC) technique was used to condense chromosomes in the G2 phase of the cell cycle. Results showed that the frequency of unrejoined chromosome breaks was higher after high-LET radiation, and the ratio of unrejoined to misrejoined chromosome breaks increased steadily with LET up a peak value at 440 keV/μm.
- 日本放射線影響学会の論文
著者
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KAWATA Tetsuya
Department of Radiology, Graduate School of Medicine, Chiba University
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CUCINOTTA Francis
NASA Lyndon B Johnson Space Center, Radiation Biophysics Laboratory Houston TX
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GEORGE Kerry
NASA Lyndon B Johnson Space Center, Radiation Biophysics Laboratory Houston TX
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FURUSAWA Yoshiya
National Institute of Radiological Sciences
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FUTAMI Yasuyuki
Shizuoka Cancer Center
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Futami Yasuyuki
茨城県立医療大学 放射線技術科学科
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Futami Yasuyuki
The Institute Of Physics University Of Tsukuba
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Futami Yasuyuki
Dvisin Of Accelerator Physics And Engineering National Institite Of Radiological Sciences Chiba Japa
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George Kerry
Nasa Lyndon B Johnson Space Center Radiation Biophysics Laboratory Houston Tx
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George Kerry
米国
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George Kerry
Nasa Lyndon B. Johnson Space Center Radiation Biophysics Laboratory
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WU HONGLU
Wyle Laboratories
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GEORGE KERRY
Wyle Laboratories
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KAWATA TETSUYA
Chiba University
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Furusawa Y
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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Cucinotta Francis
Nasa Lyndon B Johnson Space Center Radiation Biophysics Laboratory Houston Tx
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Kawata Tetsuya
Department Of Radiology Graduate School Of Medicine Chiba University
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Wu Honglu
Wyle Life Sciences:nasa Johnson Space Center
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