Number of Fe Ion Traversals Through a Cell Nucleus for Mammalian Cell Inactivation Near the Bragg Peak

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概要

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• HeLa and CHO-K1 cells were irradiated with Fe ions (1.14MeV/nucleon) near the Bragg peak to determine how many ion traversals through a cell nucleus are necessary to induce cell inactivation. The ion traversals through a cell nucleus were visualized by immunostaining the phosphorylated histone H2AX (γ-H2AX), as an indicator of DNA double strand breaks (DSBs), to confirm that DSBs are actually induced along every Fe ion traversal through the nucleus. The survival curves after irradiation with Fe ions decreased exponentially with the ion fluence without a shoulder. The inactivation cross sections calculated from the slope of the survival curves and the standard errors were 96.9±1.8 and 57.9±5.4μm^2 for HeLa and CHO-K1 cells, respectively, corresponding to 0.442 and 0.456 of the mean value of each cell nucleus area. Taking the distribution of the cell nucleus area into consideration with an equation proposed by Goodhead et al. (1980), which calculates the average number of lesions per single ion track through the average area of a sensitive organelle (mainly nucleus), these two ratios were converted to 0.705 and 0.659 for HeLa and CHO-K1 cells, respectively. These ratios were less than one, suggesting that the average numbers of lethal hits per cell produced by a single ion traversal were less than one. We thus considered two possible explanations for ion traversals of more than one, necessary for cell inactivation.

• 日本放射線影響学会の論文
• 2005-12-28

著者

• MATSUMOTO Kenichi

  Department of Neurosurgery, Graduate School of Medical Sciences, Kyushu University

• NAKAJIMA Hiroshi

  Department of Earth and Space Science, Osaka University

• YASUDA Nakahiro

  National Institute of Radiological Sciences

• FURUSAWA Yoshiya

  National Institute of Radiological Sciences

• Futami Yasuyuki

  The Institute Of Physics University Of Tsukuba

• Konishi Teruaki

  Dept. Technical Support And Development National Institute Of Radiological Sciences

• NATSUME Toshiyuki

  Department of Surgery, Funabashi Municipal Medical Center

• Kakizaki Takehiko

  Dept. Of Biochem. Gunma Univ. Graduate Sch. Med.

• Sato Yukio

  National Institute Of Radiological Sciences

• KONISHI Teruaki

  Department of Life Science, Rikkyo University

• TAKEYASU Akihiro

  Department of Life Science, Rikkyo University

• ASUKA Taku

  Faculty of Maritime Sciences, Kobe University

• HIEDA Kotaro

  Department of Life Science, Rikkyo University

• MATSUMOTO Kenichi

  Dept Sci. Toho Univ.

• Sato Yukio

  National Institute Of Radiol. Sciences

• YASUDA Nakahiro

  Natl Inst Radiol Sci

• Natume Toshiyuki

  Department Of Life Science Rikkyo University

• Natsume Toshiyuki

  Department Of Anesthesia National Cancer Center Hospital East

• Matsumoto Kenichi

  Department Of Physics Toho University

• Matsumoto Kenichi

  Department Of Neurosurgery Kyushu Rosai Hospital:(present Address)department Of Neuropathology Neuro

• HIEDA Kotaro

  Natl Inst Radiol Sci

• Furusawa Y

  Research Center For Charged Particle Therapy National Institute Of Radiological Sciences

• Asuka Taku

  Faculty Of Maritime Sciences Kobe University

• Nakajima Hiroshi

  Department Of Biochemistry Central Research Institute Maruha Corporation

• Hieda Kotaro

  Department Of Life Science Rikkyo University

• Hieda Kotaro

  Coll. Sci. Rikkyo Univ

• Hieda Kotaro

  Rikkyo University

• Kimura Takafumi

  Graduate School Of Life Sciences Tohoku Univ.

• Hieda Kotaro

  Rikkyo University College Of Science.

• Kanai Tatsuaki

  Department Of Technical Support And Development Fundamental Technology Center National Institute Of

• Kakizaki Takehiko

  Dep. Of Radiation Oncology Gunma Univ. Graduate School Of Medicine

• Matsumoto Kenichi

  Department Of Neuropathology Graduate School Of Medical Sciences Kyushu University

• Matsumoto Kenichi

  Department Of Image Based Medicine Institute Of Biomedical Research And Innovation

• Takeyasu Akihiro

  Department Of Life Science Rikkyo University

• NAKAJIMA Hiroshi

  Department of Allergy and Clinical Immunology, Chiba University Hospital

• SATO Yukio

  National Inst. Radiological Sci.

• Yasuda Nakahiro

  National Institute of Radiological Science, Chiba 263-8555, Japan

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