Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation
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概要
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In this study, whether survivin plays a direct role in mediating high-LET radiation resistance in human hepatoma cells was investigated. Small interfering RNA (siRNA) targeting survivin mRNA was designed and transfected into human hepatoma HepG2 cells. Real-time PCR and western blotting analyses revealed that survivin expression in HepG2 cells decreased at both transcriptional and post-transcriptional levels after treatment with survivin-specific siRNA. Caspase-3 activity was determined with a microplate reader assay as well. Following exposure to high-LET carbon ions, a reduced clonogenic survival effect, increased apoptotic rates and caspase-3 activity were observed in the cells treated with the siRNA compared to those untreated with the siRNA. The cells with transfection of the survivin-specific siRNA also increased the level of G2/M arrest. These results suggest that survivin definitely plays a role in mediating the resistance of HepG2 cells to high-LET radiation and depressing survivin expression might be useful to improve the therapeutic efficacy of heavy ions for radioresistant solid tumors.
- 2011-05-16
著者
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Furusawa Yoshiya
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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Futami Yasuyuki
茨城県立医療大学 放射線技術科学科
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Futami Yasuyuki
The Institute Of Physics University Of Tsukuba
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Furusawa Yoshiya
高エネルギー加速器研究機構物質構造科学研究所
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Li Ping
Key Lab. Of Heavy Ion Radiation Biology And Medicine Of Chinese Acad. Of Sciences
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DAI Zhongying
Institute of Modern Physics, Chinese Academy of Sciences
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JIN Xiaodong
Institute of Modern Physics, Chinese Academy of Sciences
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LI Qiang
Institute of Modern Physics, Chinese Academy of Sciences
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Gong Li
Institute Of Modern Physics Chinese Academy Of Sciences
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Li Ping
Institute Of Modern Physics Chinese Academy Of Sciences
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Li Qiang
Institute Of Modern Physics Chinese Academy Of Sciences
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Matsumoto Yoshitaka
Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences
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Jin Xiaodong
Institute Of Modern Physics Chinese Academy Of Sciences
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Dai Zhongying
Institute Of Modern Physics Chinese Academy Of Sciences
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WU Qingfeng
Institute of Modern Physics, Chinese Academy of Sciences
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MATSUMOTO Yoshitaka
Research Center for Heavy-ion Therapy, National Institute of Radiological Sciences
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HAO Jifang
Institute of Modern Physics, Chinese Academy of Sciences
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Hao Jifang
Institute Of Modern Physics Chinese Academy Of Sciences
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Wu Qingfeng
Institute Of Modern Physics Chinese Academy Of Sciences
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Furusawa Yoshiya
Div. Of Accelerator Res. Natl. Inst. Of Radiol. Sci.
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