Alamine Racemase Gene Fragments as Probes for Detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in Foods

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概要

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• Alanine recemase gene fragments containing non-conserved regions of the gene were evaluated as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods. The gene fragments were amplified from genomic DNA of each bacterium by polymerase chain reaction with degenerate oligonucleotide primers, and labeled with digoxigenin as probes for detecting each bacterium. Foods and bacteria were treated at 25℃ for 10 min in 0.1 N NaOH containing 0.5% SDS before being directly spotted onto nylon membranes for DNA hybridization. When the specificities of the probes were analyzed using a total of 86 strains(23 genera and 65 species)of bacteria including 29 Bacillus strains(20 species), each probe was specific for the respective target bacteria. A variety of foods inoculated with B. stearothermophilus or B. psychrosaccharolyticus were positive as determined by hybridization with the respective probe, whereas uninoculated foods were negative. The alanine recemase gene fragments could be used as specific probes for detecting B. stearothermophilus and B. psychrosaccharolyticus in foods.

• 社団法人日本生物工学会の論文
• 1998-06-25

著者

• OKUBO Yoko

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Teikyo University

• Kumagai H

  Kyoto Univ. Kyoto Jpn

• YOKOIGAWA Kumio

  Department of Food Science and Nutrition, Nara Women's University

• KAWAI Hiroyasu

  Department of Food Science and Nutrition, Nara Women's University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• 河合 弘康

  関西女子短期大学

• Kawai Hiroyasu

  Department Of Food Science And Nutrition Nara Women's University

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Okubo Y

  Department Of Pharmacology Faculty Of Pharmaceutical Sciences Teikyo University

• Okubo Yoko

  Department Of Ecological Engineering Toyohashi University Of Technology

• Kumagai Hidehiko

  Graduate School Of Biostudies Kyoto University

• Yokoigawa K

  Nara Women's Univ. Nara Jpn

• Yokoigawa Kumio

  Department Of Food Science And Nutrition Nara Women's University

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