Evaluation of an Alanine Racemase Gene as an Indicator for the Detection of Various Escherichia coli : Reactivity of the Gene Fragment with Various E. coli O157 : H7 Isolates
スポンサーリンク
概要
- 論文の詳細を見る
We determined whether various Escheichia coli O157 : H7 isolates could be detected using a probe, an alanine recemase gene (dadX) fragment, specific to E. coli. One hundred strains of E. coli O157 : H7 were isolated from fecal samples of healthy cattles and human patients with diarrhea and from a leg of beef. Most isolates (95 strains) produced both verocytotoxin 1 (VT1,Shiga-like toxin I) and verocytotoxin 2 (VT2,Shiga-like toxin II), whereas four isolates produced only VT2. Although neight VT1 nor VT2 was detected in one isolated under the conditions used, the VT2 gene was detected. When all of the isolates were analyzed by a DNA hybridization method involving the use of the probe, positive signals were obtained from all isolates. The DNA hybridization method used for detection of E. coli is considered to detect various E. coli O157 : H7 isolates which differ in the types of verocytotoxin they produce.
- 社団法人日本生物工学会の論文
- 1997-11-25
著者
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Imai Shunsuke
Nara Prefectural Institute Of Public Health
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OKUBO Yoko
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Teikyo University
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Kumagai H
Kyoto Univ. Kyoto Jpn
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YOKOIGAWA Kumio
Department of Food Science and Nutrition, Nara Women's University
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KAWAI Hiroyasu
Department of Food Science and Nutrition, Nara Women's University
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Kumagai Hidehiko
Faculty Of Agriculture Kyoto University
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Kumagai Hidehiko
Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.
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Kumagai Hidehiko
Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University
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Umesako S
Nara Prefectural Public Health Lab. Nara Jpn
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Umesako Seiichi
Nara Prefectural Public Health Laboratory
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河合 弘康
関西女子短期大学
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Kawai Hiroyasu
Department Of Food Science And Nutrition Nara Women's University
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NAKAHIRA MAYUMI
Department of Food Science and Nutrition, Nara Women's University
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Nakahira Mayumi
Department Of Food Science And Nutrition Nara Women's University
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Kumagai H
Department Of Agricultural Chemistry The University Of Tokyo
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Okubo Y
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Teikyo University
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Okubo Yoko
Department Of Ecological Engineering Toyohashi University Of Technology
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Kumagai Hidehiko
Graduate School Of Biostudies Kyoto University
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Yokoigawa K
Nara Women's Univ. Nara Jpn
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Yokoigawa Kumio
Department Of Food Science And Nutrition Nara Women's University
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Imai Shunsuke
Nara Prefectural Institute For Hygiene And Environment
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Umesako Seiichi
Nara Prefectural Institute Of Public Health
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