Autotrophic Growth of a Hyphomicrobium sp. and Its Hydrogenase Activity
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概要
- 論文の詳細を見る
Autotrophic growth was observed in a strain of Hyphomicrobium sp. capable of utilizing methanol. The organism was grownon a mineral salt mediumunder the gas phase of H2-O2-CO2 (66% : 21% : 13%). As nitrogen source, NH4+ was essential and NO3- was not useful for autotrophic growth. The specific growth rate μ was measured at various concentration of O2, and growth was observed at high O2 concentration (about 35-40%). Hydrogenase activity was assayed by colorimetric and electrochemical methods. Methylene blue and benzylviologen were reduced by the colorimetric method in the presence of a cell free extract with H2 gas. The electric current in redox dye reduction was detected by an enzyme-fuel-cell. The pH dependency of the hydrogenase activity was observed in both assay methods.
- 社団法人 日本農芸化学会の論文
著者
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TONOMURA KENZO
Fermentation Chemistry Laboratory, College of Agriculture, University of Osaka Prefecture
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Tomizuka Noboru
Fermentation Research Institute
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Kamibayashi Akira
Fermentation Research Institute
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Uebayasi Masami
Fermentation Research Institute Agency Of Industrial Science And Technology
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