Electric Field Fusion of Lactobacillus plantarum

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概要

• 論文の詳細を見る

• Protoplast fusion of Lactobacillus plantarum ATCC10012 was examined with an electric field fusion method using its double mutants having drug resistance and auxotrophic marker genes. Under the conditions of pulse width of 150 μs and an electric field intensity of 6 kV/cm, the frequency of obtained strains carrying the phenotypes from both mutants was highest (6.2×10^<-7>). The frequency was at least one order higher than the reversion frequencies and that of conjugation. These results suggest the completion of protoplast fusion.

• 社団法人日本農芸化学会の論文
• 1996-06-23

著者

• Himejima Masaki

  Advanced Technology Laboratory Kubota Corporation

• Sakanaka Kazunobu

  Advanced Technology Laboratory Kubota Corporation

• SHIRAKAWA Noboru

  Advanced Technology Laboratory, Kubota Corporation

• Shirakawa Noboru

  Advanced Technology Laboratory Kubota Corporation

関連論文

• Chromosomal Deletion or Rearrangement in Chimeric Hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus Obtained by Cell Fusion
• Introduction of Mitochondria into Respiratory-Deficient Mutant of Yeast and Improvement of Thermostability
• Breeding a Fermentative Yeast at High Temperature Using Protoplast Fusion
• Electric Field Fusion of Lactobacillus plantarum

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