Induction and Purification of Aspergillus oryzae 1,2-α-Mannosidase(Biological Chemistry)
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概要
- 論文の詳細を見る
The culture conditions favoring the production and the subsequent purification of Aspergillus oryzae 1,2-α-mannosidase were investigated. Baker's yeast mannan as the carbon source in liquid culture was found to induce 1,2-α-mannosidase production but to depress protein and other glycosidase productions. Better enzyme production was achieved by developing a suitable cultivation medium containing baker's yeast mannan and a nitrogen source in proper concentrations. From the culture product thus obtained, 1,2-α-mannosidase could be readily purified by the affinity chromatographic method previously developed [Tanimoto et al., J. Biochem., 99, 601 (1986)], without any other chromatographic step.
- 社団法人日本農芸化学会の論文
- 1989-04-23
著者
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Katsuragi Tohoru
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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YAMAGUCHI Haruki
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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TANIMOTO Kazuhito
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Yamaguchi H
Univ. Osaka Prefecture Osaka Jpn
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Tanimoto Kazuhito
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Yamaguchi Haruki
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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