Complete Sequence, Subunit Structure, and Complexes with Pancreatic .ALPHA.-Amylase of an .ALPHA.-Amylase Inhibitor from Phaseolus vulgaris White Kidney Beans.

スポンサーリンク

概要

• 論文の詳細を見る

• The complete amino acid sequence of a white kidney bean (Phaseolus vulgaris) α-amylase inhibitor (PHA-I), which is composed of two kinds of glycopolypeptide subunits, α and β, was established by conventional methods. The polypeptide molecular weight of PHA-I determined by the light-scattering technique, considered together with the sequence molecular weights revealed for the subunits, indicated that PHA-I has the subunit stoichiometry of (αβ)2 complex. Inhibition test of PHA-I with increasing amounts of porcine pancreatic α-amylase (PPA) suggested that an inactive 2:1 complex is formed between PPA and PHA-I. In fact, two complexes differing from each other in the molar ratio of PPA to PHA-I were separated by gel filtration, and molecular weight estimation by the light-scattering technique confirmed that they are complexes of PHA-I with one or two PPA molecules. The binding of PPA to PHA-I appeared to follow simple binomial statistics, suggesting that two binding sites on PHA-I are independent and of high affinity for PPA.

• 社団法人 日本生化学会の論文

著者

• Arakawa Tsutomu

  Amgen Inc.

• Hayashi Kaeko

  Department Of Chemistry And Materials Technology Faculty Of Engineering And Design Kyoto Institue Of

• Kasahara Keiji

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• PHILO John

  Amgen Inc.

• Hara Saburo

  Department Of Applied Biology Kyoto Institute Of Technology

• Yamaguchi Haruki

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

関連論文

• Recent progress of seismic observation networks in Japan : Hi-net, F-net, K-NET and KiK-net
• Deep Drilling at the Arc-arc Collision Zone in the Yamakita Area, Central Honshu, Japan
• Seismic Reflection Profiling in the Kanto and Kinki Metropolitan Areas, Japan
• Interaction of Subtilisin BPN' and Recombinant Fungal Protease Inhibitor F from Silkworm with Substituted P_1 Site Residues
• Cloning and Nucleotide Sequence of the Gene Encoding a Serine Proteinase Inhibitor Named Marinostatin from a Marine Bacterium, Alteromonas sp. Strain B-10-31
• Fructose 1,6-Bisphosphate Aldolase Is a Heparin-Binding Protein
• Expression and Purification of Cytokine Receptor Homology Domain of Human Granulocyte-Colony Stimulating Factor Receptor in Escherichia coli
• Distribution of the Kunitz and the Bowman-Birk Family Proteinase Inhibitors in Leguminous Seeds(Biological Chemistry)
• Cloning and Expression of the Momordica charantia Trypsin Inhibitor II Gene in Silkworm by using a Baculovirus Vector
• Attenuation of glial scar formation in the injured rat brain by heparin oligosaccharides
• Enhancement of Heme-Induced Membrane Damage by the Anti-malarial Clotrimazole : the Role of Colloid-Osmotic Forces (Medicinal Chemistry)
• Neutralization of Toxic Heme by Plasmodium Falciparum Histidine-Rich Protein 2
• Expression of YAV Proteins and Vaccination against Viral Ascites among Cultured Juvenile Yellowtail
• Importance of 6-O-Sulfate Groups of Glucosamine Residues in Heparin for Activation of FGF-1 and FGF-2
• Mutual Stabilization of Detergent Resistant Subtilisin and Heat Stable Streptomyces Subtilisin Inhibitor by Complex Formation(Biological Chemistry)
• Identification of Essential Amino Acid Residues of an α-Amylase Inhibitor from Phaseolus vulgaris White Kidney Beans
• A New Family of Serine Protease Inhibitors (Bombyx Family) as Established from the Unique Topological Relation between the Positions of Disulfide Bridges and Reactive Site
• A Chimera-Like α-Amylase Inhibitor Suggesting the Evolution of Phaseolus vulgaris α-Amylase Inhibitor
• Dimerization of Grunulocyte-Colony Stimulating Factor Receptor: The Ig Plus CRH Construct Granulocyte-Colony Stimulating Factor Receptor Forms a 2:2 Complex with a Ligand
• Structural Characterization of an α-Amylase Inhibitor from a Wild Common Bean (Phaseolus vulgaris): Insight into the Common Structural Features of Leguminous α-Amylase Inhibitors
• Complete Sequence, Subunit Structure, and Complexes with Pancreatic α-Amylase of an α-Amylase Inhibitor from Phaseolus vulgaris White Kidney Beans
• Stabilization of Human Recombinant Erythropoietin through Interactions with the Highly Branched N-Glycans^1
• Trypsin and Elastase Inhibitors from Bitter Gourd (Momordica charantia LINN.) Seeds: Purification, Amino Acid Sequences, and Inhibitory Activities of Four New Inhibitors
• Expression of Bombyx Family Fungal Protease Inhibitor F from Bombyx mori by Baculovirus Vector
• Identification of the Positions of Disulfide Bonds of Chitinase from a Marine Bacterium,Alteromonas sp. Strain O-7
• The Primary Structure of Pepstatin-Insensitive Carboxyl Proteinase Produced by Pseudomonas sp. No. 101
• Amino Acid Sequences of Trypsin Inhibitors from Oriental Pickling Melon (Cucumis melo L. var. Conomon MAKINO) Seeds
• N-Glycans Protect Proteins from Protease Digestion through Their Binding Affinities for Aromatic Amino Acid Residues^1
• Binding Affinity of N-Glycans for Aromatic Amino Acid Residues: Implications for Novel Interactions between N-Glycans and Proteins
• Promotion of Polypeptide Folding by interactions with Asn-Glycans
• Purification and Characterization of Fibroinase, a Cathepsin L-Like Cysteine Proteinase, from the Silk Gland in the Fourth Instar Bombyx mori Larva at the Fourth Molt Period, Stage D_2
• A Novel Endo-β-N-acetyl-glucosaminidase from Shoots of Phyllostachys heterocycla var. pubescens(Biological Chemistry)
• Fractionation of polyclonal antibodies to fragments of a neuroreceptor using three increasingly chaotropic solvents
• Assignment of the Absolute Configuration of Partially Methylated Galactoses by Combined Gas-liquid Chromatography-Mass Spectrometry
• A Novel Regioselective Desulfation Method Specific to Carbohydrate 6-Sulfate Using Silylating Reagents
• Regioselectivity in Sulfation of Galactosides by Sulfuric Acid and Dicyclohexylcarbodi-imide
• Leupeptins Produced by the Marine Alteromonas sp. B-10-31
• Purification and Characterization of Serine Proteinase Inhibitors form Gourd (Lagenaria leucantha RUSBY var. Gourda MAKINO) Seeds
• Thermal Denaturation of Subtilisin-SSI Complex Analyzed by SDS-PAGE(Biological Chemistry)
• Induction and Purification of Aspergillus oryzae 1,2-α-Mannosidase(Biological Chemistry)
• Intersubunit Cross-linking Study of the Subunit Structure of an α-Amylase Inhibitor from Phaseolus vulgaris White Kidney Bean
• A Chaperone-Like Function of Intramolecular High-Mannose Chains in the Oxidative Refolding of Bovine Pancreatic RNase B^1
• Topological and Functional Characterization of the N-Glycans of Soybean(Glycine max) Agglutinin^1
• Isolation and Characterization of the Subunits of a Heat-labile α-Amylase Inhibitor from Phaseolus vulgaris White Kidney Bean
• Reconstitution of Phaseolus vulgaris α-Amylase Inhibitor from Isolated Subunits
• A STUDY ON THE PHARMACOLOGICAL ACTION OF LAUDOLISSIN
• Chemical synthesis of fully active and heat-stable fragments of heat-stable enterotoxin of enterotoxigenic Escherichia coli strain 18D.
• PHARMACOLOGICAL STUDIES ON THE FUNCTION OF THE EXTRAPYRAMIDAL SYSTEM:REPORT NO. 2 MECHANISM OF THE APPEARANCE OF TREMOR DUE TO EXTRAPYRAMIDAL POISONS
• A new method for protein sequence analysis using edman-degradation, field-desorption mass spectrometry and computer calculation. Sequence determination of the N-terminal BrCN fragment of Streptomyces erythraeus lysozyme.
• Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli.
• Heat-stable enterotoxin (STh) of human enterotoxigenic Escherichia coli (strain SK-1). Structure-activity relationship.
• PHARMACOLOGICAL STUDIES IN THE FUNCTIONS OF THE EXTRAPYRAMIDAL SYSTEM:REPORT NO. 1
• ON THE SPECIES DIFFERENCE OF THE APPEARANCE OF BLOOD PRESSURE ACTION OF LOBELINE IN ANIMALS
• Complete Sequence, Subunit Structure, and Complexes with Pancreatic .ALPHA.-Amylase of an .ALPHA.-Amylase Inhibitor from Phaseolus vulgaris White Kidney Beans.
• Identification of Essential Amino Acid Residues of an .ALPHA.-Amylase Inhibitor from Phaseolus vulgaris White Kidney Beans.
• Amino acid sequence of glutathione S-transferase b from guinea pig liver.
• N-Glycans Protect Proteins from Protease Digestion through Their Binding Affinities for Aromatic Amino Acid Residues.
• Effect of Antifungal Azoles on the Heme Detoxification System of Malarial Parasite.
• Neutralization of Toxic Heme by Plasmodium Falciparum Histidine-Rich Protein 2

もっと見る 閉じる

スポンサーリンク