A new method for protein sequence analysis using edman-degradation, field-desorption mass spectrometry and computer calculation. Sequence determination of the N-terminal BrCN fragment of Streptomyces erythraeus lysozyme.
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概要
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A new method is described for protein sequence analysis. The principle is realized as follows: 1) determination by field-desorption mass spectrometry of the molecular weights of the constituents in peptide mixtures prepared from a polypeptide or protein by specific cleavage methods, 2) quantitation of the 3-phenyl-2-thiohydantoin derivatives of amino acids liberated successively from the constituent peptides in 1) by Edman-degradation, and 3) determination of amino acid sequences with a computer from the data obtained in 1) and 2). The method was applied to sequence determination of a polypeptide of unknown structure, the <I>N</I>-terminal BrCN fragment of <I>Streptomyces erythraeus</I> lysozyme.
- 公益社団法人 日本化学会の論文
著者
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Katakuse Itsuo
Department Of Physics Graduate School Of Science Osaka University
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Hong Yeong-Man
Institute for Protein Research, Osaka University
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Shimonishi Yasutsugu
Institute For Protein Research Osaka University
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Hara Saburo
Department Of Applied Biology Kyoto Institute Of Technology
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Hara Saburo
Department of Chemistry, Faculty of Science, Osaka University
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