Regeneration to the native form of hen egg-white lysozyme from its protected derivatives.
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概要
- 論文の詳細を見る
The following derivatives of hen egg-white lysozyme were prepared from native hen egg-white lysozyme, 2-chlorobenzyloxycarbonyl-lysozyme, <I>S</I>-(4-methylbenzyl)-reduced-lysozyme, and 2-chlorobenzyloxycarbonyl-<I>S</I>-(4-methylbenzyl)-reduced-lysozyme. Treatment of these derivatives with anhydrous liquid hydrogen fluoride for 90 min at 0 °C yielded fully active lysozyme with recoveries, in order, of 23, 12, and 7.4%. The properties of the lysozyme recovered were identical with those of native hen egg-white lysozyme, and crystals of this material were indistinguishable from those of native enzyme.
- 公益社団法人 日本化学会の論文
著者
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AIMOTO Saburo
Institute for Protein Research, Osaka University
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Shimonishi Yasutsugu
Institute For Protein Research Osaka University
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Aimoto Saburo
Institude For Protein Research Osaka University
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