Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli.
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概要
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A peptide with the amino acid sequence proposed for a heat-stable enterotoxin (ST<SUB>h</SUB>) produced by a human strain SK-1 of enterotoxigenic <I>Escherichia coli</I> was synthesized by a conventional method. Synthetic ST<SUB>h</SUB> evoked fluid secretion in suckling mice at a dose of 0.8 ng, which is similar to the effective dose of native ST<SUB>h</SUB>. The fluid secretion was completely inhibited by antisera raised against the native toxin. Furthermore, synthetic ST<SUB>h</SUB> showed the same <SUP>1</SUP>H-NMR spectrum and heat-stability as native ST<SUB>h</SUB>.
- 公益社団法人 日本化学会の論文
著者
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Shimonishi Yasutsugu
Institute For Protein Research Osaka University
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Aimoto Saburo
Institude For Protein Research Osaka University
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Yoshimura Shoko
Institute For Protein Research Osaka University
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Hara Saburo
Department Of Applied Biology Kyoto Institute Of Technology
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Miwatani Toshio
Research Institute For Microbial Diseases Osaka University
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Ikemura Haruo
Institute for Protein Research, Osaka University
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Takeda Tae
Research Institute for Microbial Diseases, Osaka University
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Takeda Yoshifumi
Institute for Protein Research, Osaka University
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