Chemical synthesis of fully active and heat-stable fragments of heat-stable enterotoxin of enterotoxigenic Escherichia coli strain 18D.
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概要
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Two shorter peptides of a heat-stable enterotoxin (ST<SUB>p</SUB>), which is produced by enterotoxigenic <I>Escherichia coli</I> strain 18D, were prepared by two different procedures; i) chemical synthesis and ii) Edman-degradation of ST<SUB>p</SUB>. These peptides (designated as ST<SUB>p</SUB>(4–18) and ST<SUB>p</SUB>(5–18)) corresponded to 15 and 14 amino acid residues in the sequence of ST<SUB>p</SUB> without three and four residues, respectively, of the N-terminus. Peptides ST<SUB>p</SUB>(4–18) and ST<SUB>p</SUB>(5–18) prepared by chemical synthesis had the same biological and physicochemical properties as the peptides obtained by the Edman method. Furthermore, they showed almost the same toxicity as native and synthetic ST<SUB>p</SUB>, and their toxicity was neutralized by anti-native ST<SUB>p</SUB> antisera. Synthetic ST<SUB>p</SUB>(4–18) had similar heat-stability to ST<SUB>p</SUB>, but synthetic ST<SUB>p</SUB>(5–18) was much more heat-stable. These findings suggest that the sequence from the Cys residue near the N-terminus to the C-terminal residue is extremely important for the unique characters of toxicity and heat-stability of the toxin.
- 公益社団法人 日本化学会の論文
著者
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Takao Toshifumi
Institute For Protein Research Osaka University
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Takeda Yoshifumi
Research Institute International Medical Center Of Japan
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Shimonishi Yasutsugu
Institute For Protein Research Osaka University
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Aimoto Saburo
Institude For Protein Research Osaka University
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Yoshimura Shoko
Institute For Protein Research Osaka University
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MIWATANI Toshio
Research Institute for Microbial Diseases, Osaka University
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Hara Saburo
Department Of Applied Biology Kyoto Institute Of Technology
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Miwatani Toshio
Research Institute For Microbial Diseases Osaka University
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Ikemura Haruo
Institute for Protein Research, Osaka University
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Takeda Tae
Research Institute for Microbial Diseases, Osaka University
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Hara Saburo
Department of Chemistry, College of Science, Osaka University
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