A Proteomic Analysis of Leaf Sheaths from Rice.
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概要
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The proteins extracted from the leaf sheaths of rice seedlings were separated by 2-D PAGE, and analyzed by Edman sequencing and mass spectrometry, followed by database searching. Image analysis revealed 352 protein spots on 2-D PAGE after staining with Coomassie Brilliant Blue. The amino acid sequences of 44 of 84 proteins were determined; for 31 of these proteins, a clear function could be assigned, whereas for 12 proteins, no function could be assigned. Forty proteins did not yield amino acid sequence information, because they were N-terminally blocked, or the obtained sequences were too short and/or did not give unambiguous results. Fifty-nine proteins were analyzed by mass spectrometry; all of these proteins were identified by matching to the protein database. The amino acid sequences of 19 of 27 proteins analyzed by mass spectrometry were similar to the results of Edman sequencing. These results suggest that 2-D PAGE combined with Edman sequencing and mass spectrometry analysis can be effectively used to identify plant proteins.
- 社団法人 日本生化学会の論文
著者
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Takao Toshifumi
Institute For Protein Research Osaka University
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Matsubae Masami
Institute For Protein Research Osaka University
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Shen Shihua
Department Of Molecular Biology National Institute Of Agrobiological Sciences
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Tanaka Naoki
Department Of Bio-molecular Engineering Kyoto Institute Of Technology
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Komatsu Setsuko
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Scien
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