Effect of Abscisic Acid on Phosphatidylserine-sensitive Calcium Dependent Protein Kinase Activity and Protein Phosphorylation in Rice
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概要
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Protein kinase activity in the embryos of rice (Oryza sativa L. cv. Nipponbare) during seed soaking in water was assessed under various conditions. The histone III-S phosphorylating activity in the membrane fraction prepared from control seedlings treated with water was increased by the addition of Ca^<2+> (0.2 mM), and it increased further up on the simultaneous addition of phosphatidylserine (PS). By contrast, the activity in extracts prepared from ABA-treated seeds increased by Ca^<2+> alone and PS did not augment this increased level of activity. In vitro phosphorylation of the membrane fraction from control seeds in the presence of Ca^<2+> and PS resulted in phosphorylation of two proteins with molecular masses and isoelectric points of 40 kDa/8.9 and 40 kDa/7.6, respectively. When seeds were treated with exogenous ABA, phosphorylation in vitro of these two proteins was dependent only on the addition of Ca^<2+>. V(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) did not block phosphorylation of these proteins, which is dependent on Ca^<2+>. The varieties and quantities of phospholipids in the seeds after soaking in water containing ABA were the same as those after soaking only in water. Phosphorylation of the 40-kDa proteins in vitro in the membrane fraction from control seeds required higher Ca^<2+> concentrations (1-2 mM) compared to that observed in the membrane fraction from ABA-treated seeds.
- 社団法人日本農芸化学会の論文
- 1997-03-23
著者
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Masuda Taizo
Department Of Molecular Biology National Institute Of Agrobiological Resources
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Masuda Taizo
Department Of Soils And Fertilizers National Agriculture Research Center
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KARIBE Hideji
Department of Molecular Biology, National Institute of Agrobiological Resources
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Komatsu Setsuko
Department Of Molecular Biology National Institute Of Agrobiological Resources
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Karibe Hideji
Department Of Molecular Biology National Institute Of Agrobiological Resources
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Komatsu Setsuko
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Scien
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