Influence of Anhydrous Hydrogen Fluoride on Hen Egg-White Lysozyme. I. Effects on Native Hen Egg-White Lysozyme

スポンサーリンク

概要

• 論文の詳細を見る

• The influences of anhydrous hydrogen fluoride on the native form of hen egg-white lysozyme under standard conditions of peptide synthesis were investigated by measuring the enzymatic activities of materials recovered from mixtures of anhydrous hydrogen fluoride and hen egg-white lysozyme. The enzymatic activity of hen egg-white lysozyme gradually decreased on incubation with anhydrous hydrogen fluoride at a given temperature. After incubation of the mixture at 0 °C for 60 min, at least three fractions could be separated by gel-filtration on Sephadex G-50. One of these was eluted in the same position as native lysozyme and was fully active enzymatically. Its ultra-violet, and circular dichroism absorptions were identical with those of native lysozyme, and the tetragonal crystals obtained from this fraction could not be distinguished from those of native lysozyme.

• 公益社団法人 日本化学会の論文

著者

• Shimonishi Yasutsugu

  Institute For Protein Research Osaka University

• Aimoto Saburo

  Institude For Protein Research Osaka University

関連論文

• Amino Acid Sequence of αk Substance, a Mating Pheromone of Saccharomyces kluyveri
• Development of a Method for the Preparation of Peptide Thioesters by an Fmoc Solid Phase Method
• Synthesis of Cysteine-Containing Polypeptide Using a Peptide Thioester Containing a Cys(Acm) Residue
• Polypeptide Synthesis Using Cys(Acm)-Containing Peptide Thioester as Building Block
• Synthesis of a Partial Sequence of Site-Specifically Methylated Histone H3
• Peptide Purification Using α-Ketoacyl Group Chemistry
• Preparation of a Peptide Thioester by the Fmoc-Solid Phase Method via an On-Resin N-S Acyl Shift
• Chemical Ligation Using a Photoremovable Auxiliary
• Interaction of Synthetic J Protein of Bacteriophage ΦK with Its Circular Single-Stranded DNA
• Synthesis of Transmembrane Protein, F_1F_0 ATP Synthase Subunit c, by Thioester Method
• Chemical Synthesis of H^+-ATP Synthase Subunit c
• Peptide Synthesis Mediated by Thiolsubtilisin Using Peptide Thioester as Building Block
• The N-Terminal Helical Segments Modulate Structure and Lipid Interaction of Human Apolipoprotein A-I
• Chemical Synthesis of the Extracellular Juxtamembrane-Transmembrane Region of the Thrombopoietin Receptor
• Peptide Thioester Synthesis Based on an N-S Acyl Shift Reaction
• 2P332 Interaction of Arrestin with Phosphorylated Rhodopsin Cterminus Peptide(42. Sensory signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Studies on Structure-Activity Relationship of HTLV-1 Protease Inhibitors
• Solid-Phase Syntheses of Olefin-Containing Protease Inhibitors Using Horner-Emmons Reaction
• Synthesis of Opioid Receptor Like 1 (251-370) by Combining the Native Chemical Ligation and the Thioester Method
• Synthesis of Olefin-Containing Cyclic Peptide Library Using the Solid-Phase Horner-Emmons Reaction
• Solid-Phase Peptide Synthesis Using N^α-Fmoc(2-F)-Amino Acid and Its Application to the Synthesis of Phosphorylated Peptide Thioesters
• Total Synthesis of Human T-cell Leukemia Virus Type-1 Protease via the Thioester Method
• Synthesis of a Molt-Inhibiting Hormone of the American Crayfish, Procambarus clarkii and Determination of Its Disulfide Linkage
• Synthesis of Phosphorylated Polypeptide Using an Expressed Peptide as a Building Block
• Changes in the Amounts of the Molt-Inhibiting Hormone in Sinus Glands during the Molt Cycle of the American Crayfish, Procambarus clarkii : Endocrinology
• Synthesis of a Molt-Inhibiting Hormone of the American Crayfish, Procambarus clarkii, and Determination of the Location of Its Disulfide Linkages^1
• Use of an Expressed Peptide Segment as a Building Block for the Preparation of Phosphorylated Polypeptide via the Thioester Method
• Synthesis of a Phosphorylated Polypeptide by a Thioester Method
• SYNTHESIS OF A MOLT - INHIBITING HORMONE IN THE AMERICAN CRAYFISH PROCAMBARUS CLARKII BY NATIVE CHEMICAL LIGATION
• Synthesis of Cysteine-Containing Polypeptide Using Peptide Thioester : Preparation of a Molt-Inhibiting Hormone from the American Crayfish, Procambarus clarkii
• A Novel Modification of the Lysine Residue at Position 12 of Histone H4 in Starfish Sperm
• Peptide Ligation Using a Building Block Having a Cysteinyl Prolyl Ester (CPE) Autoactivating Unit at the Carboxy Terminus
• Sequential Peptide Ligation by the Combination of the Thioester Method and Extended Chemical Ligation
• N-S Acyl Shift Reaction on Peptide Backbone and Its Application to Preparation of Peptide Thioesters
• Peptide Ligation That Involves an Intramolecular N to S Acyl Shift Reaction
• Generation of an S-Peptide via an N-S Acyl Shift Reaction in a TFA Solution
• Preparation of Peptide Thioesters Based on an N-S Acyl Shift Reaction
• The N-Terminal Region of Prouroguanylin Responsible for the Folding of Uroguanylin
• Folding Mechanism of Guanylyl Cyclase-Activating Peptides and its Precursor Protein
• The Role of Propeptide in the Folding Pathway of Uroguanylin
• Xenopus Eggs Express an Identical DNA Methyltransferase, Dnmtl, to Somatic Cells
• Molecular Mechanism of Liposome Membrane Fusion Induced by Amphipathic Helical Peptides
• Isolation of Bioactive Peptides from Wamide-Peptide Libraries Synthesized with a Multipeptide Synthesizer
• Isolation of Neurotensin-related Bioactive Pentapeptides from Peptide Libraries Synthesized with a Multipeptide Synthesizer
• Preparation of S-Protected Cysteine-Containing Peptide Thioester and Its Use for the Synthesis of the Barnase-Like Domain in DNA-Directed RNA Polymerase II of Saccharomyces cerevisiae
• Synthesis of β-Peptides utilizing C-Glycosyl β-Amino Acid
• Peptide Ligation Using a Photoremovable Auxiliary
• Characterization of the Ligand-Binding Specificities of Integrin α3β1 and α6β1 Using a Panel of Purified Laminin Isoforms Containing Distinct α Chains
• Structure of the Transmembrane : Juxtamembrane Region of Amyloid Precursor Protein
• Structure of the Membrane-Reconstituted Transmembrane-Juxtamembrane Peptide, Neu(647-693), and Its Interaction with Ca^/Calmodulin
• Structural Analysis of the Transmembrane-Juxtamembrane Region of FGFR3
• Structure of the Transmembrane - Juxtamembrane Sequence of EGFR and Its Interaction with Ca^/Calmodulin
• Characterization of Lipid-Binding Domains in Human Serum Amyloid A Using Its Fragment Peptides
• Peptide Purification Using The Selective Reaction Between Aminooxy Group and Isothiocyanate Group, Followed by Edman Degradation
• Methylthio Groups for Thiol Protection that Realize the Sequential Ligation by Native Chemical Ligation Followed by Thioester Method
• 2-Nitrobenzylcarbamate-type Photocleavable Linker Having Fmoc-aminoalkyloxy Group : Preparation and Application to Peptide Purification
• Synthesis of Voltage-Dependent Proton Channel (VSOP)(121-222)
• pQVPLPRYamide Isolated from the Clam Meretrix lusoria : a Member of a Novel Molluscan Neuropeptide Family
• Structure-Activity Relation of LWamide Peptides Synthesized with a Multipeptide Synthesizer
• Structure and Action of Bioactive Peptides Isolated from the Newt, Cynops pyrrhogaster : I. A. C-type Natriuretic Peptide (CNP)
• An Antagonist of Molluscan Neuropeptide APGW amide Found in a Peptide Library
• Bioactive Peptides Isolated from An African Ancient-type Fish, Polypterus senegalus
• Structure and Action of Bioactive Peptides Isolated from the Newt, Cynops pyrrhogaster : II. Four VIP-Related Peptides and Two Neurotensin-Related Peptides
• Propeptide-Mediated Folding in a Peptide Hormone : Uroguanylin
• New Phosphoryl Protecting Groups for Synthesis of Phosphopeptides by Boc Method
• Stereoselective Synthesis and Evaluation of HTLV-1 Protease Inhibitors
• IDENTIFICATION OF THE BINDING SITE ON THE EXTRACELLULAR DOMAIN OF GUANYLYL CYCLASE C TO HEAT-STABLE ENTEROTOXIN
• Preparation of Partially Protected Peptide Thioesters Containing a Cysteine Residue and Their Segment Condensation
• A New Method for the Sequence Determination of a Peptide Mixture through Molecular Weight Measurements by Mass Spectrometry
• A Cystine Motif in A Novel Endogenous Inhibitor of Phenoloxidase
• New Redox Linker for the Preparation of Subunit c of Human F_1F_O-ATP Synthase
• Condensation of Cys(Acm)-Containing Peptide Segments with Silver Chloride as an Activator of Peptide Thioesters
• Peptide Thioester Formation by an N to S Acyl Shift Reaction at the Cysteinyl Prolyl Cysteine Position
• Synthesis of Peptide Thioester by the Cysteinyl Prolyl Ester (CPE) Method
• Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel
• The Micro Domain Essential for the Ligand-Binding of Guanylyl Cyclase C
• Precious Role of Ile^3 of Prouroguanylin in the Folding of the Mature Peptide, Uroguanylin
• Folding Mechanism of Prouroguanylin
• A Study of the Role of the N-terminal Region of Prouroguanylin in the Folding by Site-directed Mutational Analysis
• Preparation of Peptide Thioester by Fmoc Solid-Phase Method
• Peptide Ligation via Thioester Formation at the Cysteine Residue
• Mass Spectrometry of Peptides and Proteins in the Post-Genome Era
• Solid State NMR study on the Dimer Structure of the Transmembrane Region of Amyloid Precursor Protein
• Study for Serotonin Release-Modulating Action by Molluscan Neuropeptide APGWamide Using an Antagonist
• Development of Potent Peptidic Analogues of APGWGNamide ; The Antagonist of Molluscan Neuropeptide APGWamide
• Pretreatment of Silicon Emitter for the Enhancement of Ionization Efficiency in Field Desorption Mass Spectrometry
• Computation of amino acid sequences of polypeptides from masses of their constituent peptide fragments and amino acid residues released in Edman-degradation.
• A new method for carboxyl-terminal sequence analysis of a peptide using carboxypeptidases and field-desorption mass spectrometry.
• Chemical synthesis of a heat-stable enterotoxin produced by enterotoxigenic Escherichia coli strain 18D.
• Structure-activity relationship of a heat-stable enterotoxin produced by Yersinia enterocolitica.
• Regeneration to the native form of hen egg-white lysozyme from its protected derivatives.
• Chemical synthesis of fully active and heat-stable fragments of heat-stable enterotoxin of enterotoxigenic Escherichia coli strain 18D.
• Disulfide linkages in a heat-stable enterotoxin (STP) produced by a porcine strain of enterotoxigenic Escherichia coli.
• Structure-activity relationship of Escherichia coli heat-stable enterotoxin: Role of Ala residue at position 14 in toxin-receptor interaction.
• Influence of Anhydrous Hydrogen Fluoride on Hen Egg-White Lysozyme. I. Effects on Native Hen Egg-White Lysozyme
• Synthesis of a partial sequence of proinsulin using the A-chain of natural insulin. III. Synthesis of a peptide corresponding to positions 41-81 of bovine proinsulin.
• Structural requirements for the spatial structure and toxicity of heat-stable enterotoxin(STh) of enterotoxigenic Escherichia coli.
• A new method for protein sequence analysis using edman-degradation, field-desorption mass spectrometry and computer calculation. Sequence determination of the N-terminal BrCN fragment of Streptomyces erythraeus lysozyme.
• Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli.
• Heat-stable enterotoxin (STh) of human enterotoxigenic Escherichia coli (strain SK-1). Structure-activity relationship.

もっと見る 閉じる

スポンサーリンク