Investigation of a Novel Bacillus thuringiensis Gene Encoding a Parasporal Protein, Parasporin-4, That Preferentially Kills Human Leukemic T Cells
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概要
- 論文の詳細を見る
A novel gene encoding a leukemic cell-killing parasporal protein, designated parasporin-4, was cloned from an isolate of Bacillus thuringiensis serovar shandongiensis. The amino acid sequence of the parasporin-4, as deduced from the gene sequence, had low-level homologies of <30% with the established B. thuringiensis Cry proteins including the three known parasporins. When the gene was expressed in a recombinant of Escherichia coli BL21(DE3), the parasporin-4 formed intracellular inclusion bodies. Alkali-solubilized and proteinase K-activated inclusion protein exhibited strong cytotoxic activity against human leukemic T cells (MOLT-4) and weak for normal T cells, but no adverse effect on human uterus cervix cancer cells (HeLa).
- 社団法人 日本農芸化学会の論文
- 2006-12-23
著者
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MIZUKI Eiichi
Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
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OHBA Michio
Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
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Mizuki Eiichi
Biotechnology And Food Res. Inst. Fukuoka Industrial Technol. Center
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Mizuki Eiichi
Department Of Applied Genetics And Pest Management Faculty Of Agriculture Kyushu University
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Akao Tetsuyuki
Biotechnology And Food Res. Inst. Fukuoka Industrial Technol. Center
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Ohba Michio
Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University
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Ohba Michio
Graduate School Of Agriculture Kyushu University
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Ohba M
Kyushu University
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Ohba Michio
Department Of Applied Genetics And Pest Management Faculty Of Agriculture Kyushu University
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Saitoh Hiroyuki
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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Okumura Shiro
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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ISHIKAWA Tomoyuki
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
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Ishikawa Tomoyuki
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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Saito Hiroyuki
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
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- Investigation of a Novel Bacillus thuringiensis Gene Encoding a Parasporal Protein, Parasporin-4, That Preferentially Kills Human Leukemic T Cells
- Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells
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