Characteristic Behavior of Viscosity and Viscoelasticity of Aureobasidium pullulans Culture Fluid
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概要
- 論文の詳細を見る
Measurements of dynamic viscoelasticity and steady flow viscosity were made for culture fluids obtained by cultivation of Aureobasidium pullulans IAM 5060 with initial pHs of 6 and 7 and for exopolysaccharide (EPS) solutions obtained by removal of microbial cells. The molecular weight of EPS of the pH 6 culture is about 850,000, and that of the pH 7 culture is much larger. in the present study, the complex viscosity shifted to a considerably larger value than that of the steady flow viscosity. This differs from the Cox-Merz experience law which claims that there is a similarity between the angular frequency dependence of complex viscosity and the shear rate dependence of steady flow viscosity. On the other hand, the dynamic viscosity at small strain amplitude practically corresponded to the steady flow viscosity. The storage modulus of the pH 7 samples decreased markedly with a strain amplitude of more than 0.2, indicating that a dense network structure was formed. The linear region of the plot of viscoelasticity against strain amplitude was wider for the pH 6 culture, reflecting the stability of the network structure in this sample. The viscoelastic level of the EPS solution was a little greater than that of the culture fluid. This suggests that the viscoelasticity of the culture fluid is mainly caused by network structure forming among dissolved polysaccharides.
- 社団法人日本生物工学会の論文
- 2002-04-25
著者
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Toda Kiyoshi
Department of Applied Chemistry and Biotechnology, Niigata Institute of Technology
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Toda K
Univ. Tokyo Tokyo Jpn
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Toda K
Department Of Applied Chemistry And Biotechnology Niigata Institute Of Technology
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Toda Kiyoshi
Department Of Applied Chemistry And Biotechnology Niigata Institute Of Technology
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Toda K
Faculty Of Engineering Niigata Institute Of Technology
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MIYAWAKI Osato
Department of Applied Biologicao Chemistry, The University of Tokyo
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Tayama Kenji
Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.
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Toda Kiyoshi
Faculty Of Engineering Niigata Institute Of Technology
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Miyawaki O
Univ. Tokyo Tokyo Jpn
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Miyawaki Osato
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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ASAKURA TOMOKO
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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FURUSE HISAMOTO
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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ASAKURA TOMOKO
Department of Applied Biological Chemistry, University of Tokyo
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AMARI TAKESHI
Department of Information and Image Sciences, Faculty of Engineering, Chiba University
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Asakura T
Department Of Applied Biological Chemistry University Of Tokyo
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Furuse H
Univ. Tokyo Tokyo Jpn
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Furuse Hisamoto
Institute Of Applied Microbiology University Of Tokyo
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Asakura Tomoko
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Amari T
Chiba Univ. Chiba Jpn
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Amari Takeshi
Department of Applied Biological Chemistry, University of Tokyo
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