Production and Characterization of Active Soluble Human β1, 4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal β1⟶4 GlcNAc Linkage
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概要
- 論文の詳細を見る
An active and soluble human β1, 4-galactosyltransferase (β-GT) was produced in Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β-GT has a K_m value of 0.035 mM for UDP-galactose and a V_<max> of 643 × 10^3 nmol/mg/h. The enzyme catalyzes the transfer of galactose from UDP-galactose to N-linked oligosaccharides. The properties of the purified enzyme were identical to those of bovine milk β-GT.
- 社団法人日本生物工学会の論文
- 2001-01-25
著者
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Fujiyama Kazuhito
The International Center for Biotechnology, Osaka University
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SEKI Tatsuji
The International Center for Biotechnology, Osaka University
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Seki Tatsuji
The International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka University
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Maekawa Yoshihiko
Medical Research Laboratory Toyobo Co.ltd.
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SHIBATANI SHIGEO
Medical Research Laboratory, Toyobo Co.Ltd.
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NISHIGUCHI SUSUMU
Medical Research Laboratory, Toyobo Co.Ltd.
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn
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Shibatani Shigeo
Medical Research Laboratory Toyobo Co.ltd.
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Nishiguchi Susumu
Medical Research Laboratory Toyobo Co.ltd.
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