The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants(PLANT BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
When the human UDP-galactose transporter 1 gene (hUGT1) was introduced into tobacco plants, the plants displayed enhanced growth during cultivation, and axillary shoots had an altered determinate growth habit, elongating beyond the primary shoots and having a sympodial growth pattern similar to that observed in tomatoes at a late cultivation stage. The architecture and properties of tissues in hUGT1 -transgenic plants were also altered. The leaves had an increase in thickness, due to an increased amount of spongy tissue, and a higher content of chlorophyll a and b; the stems had an increased number of xylem vessels and accumulated lignin and arabinogalactan proteins (AGPs). Some of these characteristics resembled a gibberellin (GA)-responsive phenotype, suggesting involvement of GA. RT-PCR-based analysis of genes involved in GA biosynthesis suggested that the GA biosynthetic pathway was not activated. However, an increase in the proportion of galactose in polysaccharide side chains of AGPs was detected. These results suggested that because of higher UDP-galactose transport from the cytosol to the Golgi apparatus, galactose incorporation into polysaccharide side chains of AGP is involved in the gibberellin response, resulting in morphological and architectural changes.
著者
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Mohamed Khalil
Center for Gene Science, Hiroshima University
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Kajiura Hiroyuki
The International Center for Biotechnology, Osaka University
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Fujiyama Kazuhito
The International Center for Biotechnology, Osaka University
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Koike Kanae
Center for Gene Science, Hiroshima University
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Ishida Nobuhiro
Department of Environmental Security Systems, Faculty of Risk and Crisis Management, Chiba Institute
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Tanaka Nobukazu
Center for Gene Science, Hiroshima University
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Khalil Mohamed
Center For Gene Sci. Hiroshima Univ. 1-4-2 Kagamiyama Higashi-hiroshima Hiroshima 739-8527 Jpn
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Mohamed Khalil
Center For Gene Science Hiroshima University
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Tanaka Nobukazu
Center For Gene Sci. Hiroshima Univ. 1-4-2 Kagamiyama Higashi-hiroshima Hiroshima 739-8527 Jpn
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka University
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Koike Kanae
Center For Gene Science Hiroshima University
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Ishida Nobuhiro
Department Of Environmental Security Systems Faculty Of Risk And Crisis Management Chiba Institute O
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Ishida Nobuhiro
Department Of Biochemical Cell Research
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn
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Kajiura Hiroyuki
The International Center For Biotechnology Osaka University
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