Codon and Base Biases after the Initiation Codon of the Open Reading Frames in the Escherichia coli Genome and Their Influence on the Translation Efficiency.
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概要
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Nucleotide sequences around the boundaries of all open reading frames in the Escherichia coli whole genome were analyzed. Characteristic base biases were observed after the initiation codon and before the termination codon. We examined the effect of the base sequence after the initiation codon on the translation efficiency, by introducing mutations after the initiation codon of the E. coli dihydrofolate reductase (DHFR) gene, considering codon and base biases, and using in vitro and in vivo translation systems. In both assay systems, the two most frequent second codons, AAA and AAU, enhanced the translation efficiency compared with the wild type, whereas the effects of lower frequency codons were not significant. Experiments using 16S rRNA variants with mutations in the putative complementary sequence to the region downstream of the initiation codon showed that the translation efficiency of none of the DHFR mutants was affected. These results demonstrate that the statistically most frequent sequences for the second codon enhance translation efficiency, and this effect seems to be independent of base pairing between mRNA and 16S rRNA.
- 社団法人 日本生化学会の論文
著者
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Gojobori Takashi
Center For Information Biology And Ddbj National Institute Of Genetics
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Terabe Mahito
Institute For Biomolecular Science Gakushuin University
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Hori-takemoto Chie
Institute For Biomolecular Science Gakushuin University
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SATO Toru
Institute for Biomolecular Science, Gakushuin University
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Watanabe Hidemi
Human Genome Research Group Genomic Sciences Center Riken Yokohama Institute
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Miura Kin-ichiro
Institute For Biomolecular Science Faculty Of Science Gakushuin University
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