Fibril Formation by an Amphipathic α-Helix-Forming Polypeptide Produced by Gene Engineering
スポンサーリンク
概要
- 論文の詳細を見る
The α3-peptide, which comprises three repeats of the seven-residue sequence Leu-Glu-Thr-Leu-Ala-Lys-Ala, was designed to form an amphipathic α-helix with a hydrophobic surface by Leu residues and a hydrophilic surface by Glu and Lys residues, thus yielding a coiled coil or a helical bundle structure through their association. The α3-peptide was produced by gene engineering using Escherichia coli. Association to a tetramer had been demonstrated by sedimentation equilibrium analysis in a previous study. In addition to tetramerization, electron microscopic observation revealed that the α3-peptide formed“fibrils”5 to 10nm in width in 10mM potassium phosphate/0.1M KCl (pH 6.0). By increasing the salt concentration, the α3-peptide formed much larger“fibers”assembled from many“filaments”running along the long axis, each of which was thinner and longer than those observed at lower salt concentration. Hydrophobic interaction is considered to be a main force responsible for forming the fibrous structure. However, the electrostatic features of the α3-peptide seem to affect fibril assembly, since the shape and size of the fibrous structure were altered by the ionic strength of the solution. To our knowledge, this is the first report to describe formation of a fibrous structure by a de novo designed α-helix-forming peptide, and thus the α3-peptide with its simple sequence is considered to be a potential model peptide for investigating the molecular mechanisms of fibril formation by peptides or proteins.
- 日本学士院の論文
著者
-
MIURA Kin-ichiro
Institute for Biomolecular Science, Gakushuin University
-
Yazaki Kazumori
Department of Ultrastructural Research, Tokyo Metropolitan Institute of Medical Science
-
Kojima Shuichi
Institute For Biomolecular Science Gakusyuin University
-
Kojima Shuichi
Institute For Biomolecular Science Faculty Of Science Gakushuin University
-
Yazaki Kazumori
Department Of Ultrastructural Research The Tokyo Metropolitan Institute Of Medical Science
-
YOSHIDA Takao
Institute for Biomolecular Science, Faculty of Science, Gakushuin University
-
Miura Kin-ichiro
Institute For Biomolecular Science Gakushuin Univ.:research Institute Chiba Institute Of Technology.
-
KURIKI Yukino
Institute for Biomolecular Science, Gakushuin University
-
Yoshida Takao
Institute For Biomolecular Science Faculty Of Science Gakushuin University
-
Miura Kin-ichiro
Institute For Biomolecular Science Faculty Of Science Gakushuin University
-
YAZAKI Kazumori
Department of Ultrastructual Research, The Tokyo Metropolitan Institute of Medical Science
関連論文
- 2A16-4 epsilon-Poly-L-lysine(PL)分解酵素遺伝子の取得と破壊株を用いた機能解析(遺伝子工学・核酸工学,一般講演)
- 3P-1072 エナンチオ選択的イミン還元酵素の探索と特徴解析(2a酵素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
- Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
- High Performance in Refolding of Streptomyces griseus Trypsin by the Aid of a Mutant of Streptomyces Subtilisin Inhibitor Designed as Trypsin Inhibitor
- 2E09-3 炭酸固定反応を触媒する3,4-ジヒドロキシ安息香酸脱炭酸酵素の反応特性と一次構造解析(生物化学工学,一般講演)
- 1E14-4 Alcaligenes sp. IFO14130の菊酸エチルエステルと6-アミノヘキサン酸環状ダイマーに作用する加水分解酵素の関係(生物化学工学,一般講演)
- Cloning and Expression of the Catalase Gene from the Anaerobic Bacterium Desulfovibrio vulgaris (Miyazaki F)
- β-Glucosidase in the Indigo Plant : Intracellular Localization and Tissue Specific Expression in Leaves
- Purification and Characterization of a β-Glucosidase from Polygonum tinctorium, Which Catalyzes Preferentially the Hydrolysis of Indican
- EXPRESSION OF THE VOMERONASAL RECEPTOR GENES IN XENOPUS LAEVIS(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- The Mouse Putative Pheromone Receptor Was Specifically Activated by Stimulation with Male Mouse Urine
- Translational Regulation of the mRNA Encoding the Eukaryotic Translation Initiation Factor 4E in Xenopus
- Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF4E) in Initiation Complex Formation
- 1E14-3 アミダーゼが触媒するアミドの生成反応(生物化学工学,一般講演)
- Cloning and Expression of the Gene Encoding Flavodoxin from Desulfovibrio vulgaris(Miyazaki F)^1
- 1E13-4 2-ブチル-1,3-プロパンジオールの微生物交換(生物化学工学,一般講演)
- Evidence for Direct Binding of Intracellularly Distributed Ganglioside GM2 to Isolated Vimentin Intermediate Filaments in Normal and Tay-Sachs Disease Human Fibroblasts
- Primary Structure of Streptomyces griseus Metalloendopeptidase II
- 2Fp04 シアノヒドリンに作用するニトリラーゼの特性(酵素学,酵素工学,タンパク質工学,一般講演)
- 2Fp03 微生物による1,3-アダマンタンジオールの位置選択的水酸化(酵素学,酵素工学,タンパク質工学,一般講演)
- 2Fp05 Fusarium属カビのニトリラーゼの分子特性の解析(酵素学,酵素工学,タンパク質工学,一般講演)
- 1Fp09 4-ヒドロキシ安息香酸脱炭酸酵素の構造と機能発現の相関(酵素学,酵素工学,タンパク質工学,一般講演)
- 2B15-2 マンデロニトリルに作用する立体選択的ニトリラーゼ(酵素学・酵素工学・タンパク質工学,一般講演)
- 1F15-2 イソオイゲノール分解酵素の遺伝子クローニングと一次構造解析(酵素学,酵素工学,タンパク質工学,一般講演)
- 1F15-3 二相系におけるイソオイゲノールのバニリンへの微生物変換(酵素学,酵素工学,タンパク質工学,一般講演)
- 可逆的脱炭酸酵素の特性解析と芳香族カルボン酸の合成について
- 2E09-4 炭素二重結合の開裂が関与するイソオイゲノールからバニリンへの微生物変換(生物化学工学,一般講演)
- 2E09-5 イソオイゲノールからバニリンへの微生物変換 : イソオイゲノール分解酵素の精製と性質(生物化学工学,一般講演)
- Structure-Activity Analysis of an Antimicrobial Peptide Derived from Bovine Apolipoprotein A-II
- Analysis of the Isoform of Xenopus Euakryotic Translation Initiation Factor 4E
- Mitosis-specific Phosphorylation of Smooth Muscle Regulatory Light Chain of Myosin II at Ser-1 and/or -2 and Thr-9 in Sea Urchin Egg Extract
- Polysomes of eukaryotic cells observed by electron microscopy
- Transmission electron and atomic force microscopic observation of polysomes on carbon-coated grids prepared by surface spreading
- The Effects of the Side Chains of Hydrophobic Aliphatic Amino Acid Residues in an Amphipathic Polypeptide on the Formation of α Helix and Its Association
- Quantitative in vitro Renaturation of Subtilisin BPN' without the Aid of Pro-sequence
- High Frequency of SSI-Like Protease Inhibitors among Streptomyces
- 1P193 Application of polarized IR-microscope spectroscopy and solid-state NMR to structural analysis of protein fibers(6. Macromolecular assembly,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Accelerated Refolding of Subtilisin BPN' by Tertiary-Structure-Forming Mutants of Its Propeptide^1
- Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core
- 1P190 Toward creating metal binding helix fiber(6. Macromolecular assembly,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Codon and Base Biases after the Initiation Codon of the Open Reading Frames in the Escherichia coli Genome and Their Influence on the Translation Efficiency^1
- A Subtilisin Inhibitor Produced by Streptomyces bikiniensis Possesses a Glutamine Residue at Reactive Site P1
- An Endogenous Target Protease, SAM-P26, of Streptomyces Protease Inhibitor(SSI): Primary Structure, Enzymatic Characterizaion, and Its Interaction with SSI
- Low pH Facilitates Uptake of Proteins by Cells through a Non-Endocytic Pathway
- 有機溶媒耐性酵素を活用したケミカル生産(疎水性ケミカルのバイオプロダクションを目指した基盤研究)
- Fibril Formation by an Amphipathic α-Helix-Forming Polypeptide Produced by Gene Engineering
- The Effect of Hairpin DNA Fragments on Escherichia coli Poly(U)-Dependent Poly(Phe)Synthesis
- Inhibition of Translation and Progesterone-induced Maturation of Xenopus Oocytes by Expressing the Amino-terminal Portion of the Eukaryotic Translation Initiation Factor 4G(Biochemistry & Molecular Biology)
- Interference with Interaction between Eukaryotic Translation Initiation Factor 4G and Poly(A)-Binding Protein in Xenopus Oocytes Leads to Inhibition of Polyadenylated mRNA Translation and Oocyte Maturation
- Requirement of Ala Residues at g Position in Heptad Sequence of α-Helix-forming Peptide for Formation of Fibrous Structure
- Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-II
- Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins
- Introduction of Enzymatic Activity to .ALPHA.-Lactalbumin by Artificial Exon Shuffling.
- Syntheses of Optically Active α-Amino Acids from Esters of α-Keto Acids by Hydrogenolytic Asymmetric Transamination, A Solvent Effect
- Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF4E) in Initiation Complex Formation.
- Active Bacterial Flora Surrounding Foraminifera (Xenophyophorea) Living on the Deep-Sea Floor
- The Mouse Putative Pheromone Receptor Was Specifically Activated by Stimulation with Male Mouse Urine.
- Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core.
- Codon and Base Biases after the Initiation Codon of the Open Reading Frames in the Escherichia coli Genome and Their Influence on the Translation Efficiency.