5α,8α-Epidioxy-22E-ergosta-6,9(11),22-trien-3β-ol from an Edible Mushroom Suppresses Growth of HL60 Leukemia and HT29 Colon Adenocarcinoma Cells(Highlighted paper selected by Editor-in-chief,Pharmacognosy)
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概要
- 論文の詳細を見る
We purified a sterol with antitumor activity from the edible mushroom Sarcodon aspratus (BERK.) S. ITO and identified it as 5α, 8α-epidioxy-22E-ergosta-6, 9 (11), 22-trien-3β-ol (9, 11-dehydroergosterol peroxide (9 (11)-DHEP)). Purified 9 (11)-DHEP was a more effective inhibitor of HL60 leukemia cell growth and stronger apoptosis-inducer than 5α, 8α-epidioxy-22E-ergosta-6, 22-dien-3β-ol (ergosterol peroxide (EP)) that we had previously identified as an apoptosis inducer. Moreover, 9 (11)-DHEP selectively suppressed the growth of HT29 human colon adenocarcinoma cells but not WI38 normal human fibroblasts. After 5 d incubation of HT29 with 7μM 9 (11)-DHEP, the number of S phase cells decreased from 23 to 15% of total diploid cells and 17% became hypodiploid. Expression of the cyclin-dependent kinase inhibitor 1A (p21, WAF1, Cipl) (CDKN1A), which has been shown to cause cell cycle arrest and apoptosis in HT29 cells, was induced by 9 (11)-DHEP. These results suggest that 9 (11)-DHEP inhibits HT29 cell growth by inducing CDKN1A expression, thus causing cell cycle arrest and apoptosis.
- 公益社団法人日本薬学会の論文
- 2006-04-01
著者
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YOSHIDA Mitsuru
National Food Research Institute
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Ohnishi-Kameyama Mayumi
National Food Research Institute, Ministry of Agriculture, Forestry and Fishers
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SHINMOTO Hiroshi
Tamagawa University
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SHINMOTO Hiroshi
National Food Research Institute
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KOBORI Masuko
National Food Research Institute, National Agriculture and Food Research Organization
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Ohnishi-kameyama Mayumi
National Food Research Institute
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Ohnishi‐kameyama M
National Food Research Institute
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Kobori M
National Food Research Institute
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TAKEI Toshiyuki
Fukushima Prefectural Forestry Research Centre
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Yoshida Mitsuru
National Agriculture And Food Research Organization National Food Research Institute
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Kobori Masuko
National Food Research Institute
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